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Scientific article
Open access
English

Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies

Published inPractical laboratory medicine, vol. 31, e00290
Publication date2022-08
First online date2022-07-12
Abstract

Objectives: Serological assays for the presence of anti-SARS-CoV-2 antibodies are crucially needed for research and monitoring of the SARS-CoV-2 pandemic. Antibodies are reliability detected in capillary blood, a minimally invasive and cost-effective alternative to venous blood testing. However, there is a limited knowledge on feasibility of capillary blood self-sampling. This study compared the feasibility of capillary blood self-testing in people aged less than 65vs.people aged 65 or more. A secondary aim was to investigate the performance of the Hem-Col® (no additive) device compared to venous blood testing.

Design and methods: Data were collected in a prospective study in Switzerland (n = 106). Capillary blood was collected using the Hem-Col® (no additive) device. Feasibility was assessed using 1) collecting the recommended amount of capillary blood and 2) achieving all steps of capillary blood collection. A sample of 5 ml of venous blood was also collected.

Results: For the primary objective, 86.2%/62.1% of patients aged less than 65 collected the recommended amount of capillary blood/achieved all stepsvs.62.5%/39.6% of patients aged 65 or more (p = .006/p = .022). For the secondary objective, the correlation between capillary and venous blood was r = 0.992 and kappa = 1.

Conclusions: Capillary blood self-testing appeared as a feasible and reliable alternative to venous blood testing. Such alternative would improve access to serological testing and spare health care resources. However, the difference between age groups should be considered when using self-sampling devices. Help should be developed for older people, such as phone counseling or encouraging asking younger family members for help.

eng
Keywords
  • Antibodies
  • COVID-19
  • Covid-19, Coronavirus disease of 2019
  • ELISA, Enzyme-linked immunosorbent assays
  • IgG, Immunoglobulin G
  • OR, Odd-ratio
  • RT-PCR, Real-time reverse transcription–polymerase chain reaction
  • SARS-CoV-2
  • SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2
  • Serological testing
Funding
  • Fondation privée des HUG, Geneva, Switzerland. -
Citation (ISO format)
BAGGIO, Stéphanie et al. Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies. In: Practical laboratory medicine, 2022, vol. 31, p. e00290. doi: 10.1016/j.plabm.2022.e00290
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Article (Published version)
Identifiers
ISSN of the journal2352-5517
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Technical informations

Creation08/24/2022 9:52:00 AM
First validation08/24/2022 9:52:00 AM
Update time03/16/2023 8:57:38 AM
Status update03/16/2023 8:57:37 AM
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