en
Master of advanced studies
English

Screening of biological samples by SWATH acquisition and forensic toxicological applications

Master program titleMaster of Advanced Studies in Toxicology
Defense date2018-07-03
Abstract

Fast and accurate screening of biological samples is an important step in forensic toxicology for further decisions regarding possible quantification of the detected compounds in body fluids or tissues, investigations and prosecution. Based on Quadrupole Time-of-Flight (QqTOF) mass spectrometers – which are capable of acquiring complete MS and MS/MS spectra at a rate of 20 Hz or more and high mass accuracy – a new screening methodology was developed, validated and implemented in the forensic toxicological workflow. Urine samples were diluted with a mixture of water / acetonitrile / formic acid / ammonium formate (97.5 / 2.5 / 0.1% / 2.5 mM) and three internal standards were added (EME-D3, Tramadol-D3C13, THC-D3). Blood or plasma samples were prepared by protein precipitation. The samples were injected onto a core shell column (Phenomenex Kinetex C8, 50 x 2.1 mm, 2.6 μm) and analyzed on a QqTOF instrument (5600 TripleTOF, AB Sciex, Concord, Canada) with typical run times of 15 minutes from injection to injection. The methodology is based on sequential window acquisition of all theoretical fragment ions spectra (SWATH) and high resolution/high accuracy reference spectra which are used for data processing. Several forensic cases and applications are presented and the impact of this mass spectrometry based technique in the field of forensic toxicology is discussed.

eng
Keywords
  • Forensic toxicology
  • Data independent screening
  • HPLC-QqTOF
  • Sequential Windowed Acquisition of all Theoretical Fragment Ion Mass Spectra (SWATH)
  • SWATH
Citation (ISO format)
NUSSBAUMER, Susanne. Screening of biological samples by SWATH acquisition and forensic toxicological applications. 2018.
Main files (1)
Master thesis
accessLevelRestricted
Identifiers
  • PID : unige:157296
13views
1downloads

Technical informations

Creation12/10/2021 1:57:00 PM
First validation12/10/2021 1:57:00 PM
Update time03/16/2023 2:08:14 AM
Status update03/16/2023 2:08:13 AM
Last indexation01/29/2024 11:10:36 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack