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Doctoral thesis
English

The mature endosperm governs early seedling development in Arabidopsis thaliana: a focus on seedling cuticle formation

ContributorsDe Giorgi, Julien
Imprimatur date2021-10-16
Defense date2021-08-30
Abstract

In Arabidopsis mature seeds, the onset of the embryo-to-seedling transition is nonautonomously controlled, being blocked by endospermic abscisic acid (ABA) release under unfavorable conditions. Whether the mature endosperm governs additional nonautonomous developmental processes during this transition is unknown. Mature embryos have a more permeable cuticle than seedlings consistent with their endospermic ABA uptake capability. Seedlings acquire their well-sealing cuticles adapted to aerial lifestyle during germination. Endosperm removal prevents seedling cuticle formation and seed reconstitution by endosperm grafting onto embryos shows that the endosperm promotes seedling cuticle development. Grafting different endosperm and embryo mutant combinations, together with biochemical, microscopy and mass spectrometry approaches, reveals that release of tyrosylprotein sulfotransferase (TPST)-sulfated CIF2 and PSY1 peptides from the endosperm promotes seedling cuticle development. Endosperm-deprived embryos produced nonviable seedlings bearing numerous developmental defects, not related to embryo malnutrition, all restored by exogenously provided endosperm. Hence, seedling establishment is nonautonomous, requiring the mature endosperm.

eng
Keywords
  • Mature endosperm
  • Seed
  • Embryo-to-seedling transition
  • TPST
  • PSY1
  • CIF2
  • Cuticle
Research group
Citation (ISO format)
DE GIORGI, Julien. The mature endosperm governs early seedling development in Arabidopsis thaliana: a focus on seedling cuticle formation. 2021. doi: 10.13097/archive-ouverte/unige:156451
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Creation10/18/2021 10:35:00 AM
First validation10/18/2021 10:35:00 AM
Update time06/20/2023 3:10:59 PM
Status update06/20/2023 3:10:59 PM
Last indexation01/29/2024 10:58:11 PM
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