Scientific Article
previous document  unige:154520  next document
add to browser collection

Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states

Kadoch, Cigall
Williams, Robert T
Calarco, Joseph P
Miller, Erik L
Weber, Christopher M
Pulice, John L
Chory, Emma J
show hidden authors show all authors [1 - 9]
Published in Nature genetics. 2017, vol. 49, no. 2, p. 213-222
Abstract The opposition between Polycomb repressive complexes (PRCs) and BAF (mSWI/SNF) complexes has a critical role in both development and disease. Mutations in the genes encoding BAF subunits contribute to more than 20% of human malignancies, yet the underlying mechanisms remain unclear, owing largely to a lack of assays to assess BAF function in living cells. To address this, we have developed a widely applicable recruitment assay system through which we find that BAF opposes PRC by rapid, ATP-dependent eviction, leading to the formation of accessible chromatin. The reversal of this process results in reassembly of facultative heterochromatin. Surprisingly, BAF-mediated PRC eviction occurs in the absence of RNA polymerase II (Pol II) occupancy, transcription, and replication. Further, we find that tumor-suppressor and oncogenic mutant BAF complexes have different effects on PRC eviction. The results of these studies define a mechanistic sequence underlying the resolution and formation of facultative heterochromatin, and they demonstrate that BAF opposes PRC on a minute-by-minute basis to provide epigenetic plasticity.
Keywords Carcinogenesis/geneticsChromatin/geneticsDNA Replication/geneticsDNA-Binding Proteins/geneticsEpigenesisGenetic/geneticsHeterochromatin/geneticsHumansMutation/geneticsNuclear Proteins/geneticsPolycomb-Group Proteins/geneticsRNA Polymerase II/geneticsTranscriptionGenetic/genetics
PMID: 27941796
Full text
Article (Published version) (2.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group Régulation de la chromatine dans le développement du cerveau (1031)
(ISO format)
KADOCH, Cigall et al. Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states. In: Nature Genetics, 2017, vol. 49, n° 2, p. 213-222. doi: 10.1038/ng.3734

52 hits

0 download


Deposited on : 2021-09-08

Export document
Format :
Citation style :