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Title

An organelle-tethering mechanism couples flagellation to cell division in bacteria

Authors
Siwach, Manisha
Kumar, Lokesh
Palani, Saravanan
Muraleedharan, Samada
Mony, Binny M
Sanyal, Soumyajit
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Published in Developmental Cell. 2021
Abstract In some free-living and pathogenic bacteria, problems in the synthesis and assembly of early flagellar components can cause cell-division defects. However, the mechanism that couples cell division with the flagellar biogenesis has remained elusive. Herein, we discover the regulator MadA that controls transcription of flagellar and cell-division genes in Caulobacter crescentus. We demonstrate that MadA, a small soluble protein, binds the type III export component FlhA to promote activation of FliX, which in turn is required to license the conserved σ54-dependent transcriptional activator FlbD. While in the absence of MadA, FliX and FlbD activation is crippled, bypass mutations in FlhA restore flagellar biogenesis and cell division. Furthermore, we demonstrate that MadA safeguards the divisome stoichiometry to license cell division. We propose that MadA has a sentinel-type function that senses an early flagellar biogenesis event and, through cell-division control, ensures that a flagellated offspring emerges.
Identifiers
PMID: 33600766
Full text
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Supplemental data (1.6 MB) - document accessible for UNIGE members only Limited access to UNIGE
Dataset: https://data.mendeley.com/datasets/3rtdhcrwjg/1
Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE153968
Structures
Research group Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
Project
FNS: 31003A_182576
Citation
(ISO format)
SIWACH, Manisha et al. An organelle-tethering mechanism couples flagellation to cell division in bacteria. In: Developmental Cell, 2021. doi: 10.1016/j.devcel.2021.01.013 https://archive-ouverte.unige.ch/unige:149926

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Deposited on : 2021-03-02

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