en
Scientific article
English

An organelle-tethering mechanism couples flagellation to cell division in bacteria

Published inDevelopmental Cell
Publication date2021
Abstract

In some free-living and pathogenic bacteria, problems in the synthesis and assembly of early flagellar components can cause cell-division defects. However, the mechanism that couples cell division with the flagellar biogenesis has remained elusive. Herein, we discover the regulator MadA that controls transcription of flagellar and cell-division genes in Caulobacter crescentus. We demonstrate that MadA, a small soluble protein, binds the type III export component FlhA to promote activation of FliX, which in turn is required to license the conserved σ54-dependent transcriptional activator FlbD. While in the absence of MadA, FliX and FlbD activation is crippled, bypass mutations in FlhA restore flagellar biogenesis and cell division. Furthermore, we demonstrate that MadA safeguards the divisome stoichiometry to license cell division. We propose that MadA has a sentinel-type function that senses an early flagellar biogenesis event and, through cell-division control, ensures that a flagellated offspring emerges.

Citation (ISO format)
SIWACH, Manisha et al. An organelle-tethering mechanism couples flagellation to cell division in bacteria. In: Developmental Cell, 2021. doi: 10.1016/j.devcel.2021.01.013
Main files (1)
Article (Published version)
accessLevelRestricted
Secondary files (2)
Identifiers
ISSN of the journal1534-5807
241views
0downloads

Technical informations

Creation02/20/2021 8:47:00 AM
First validation02/20/2021 8:47:00 AM
Update time03/16/2023 12:11:06 AM
Status update03/16/2023 12:11:04 AM
Last indexation01/17/2024 12:38:23 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack