Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance

Papon, Nicolas; Noël, Thierry; Florent, Martine; Gibot-Leclerc, Stéphanie; Jean, Dorothée; Chastin, Christiane; Villard, Jean; Chapeland-Leclerc, Florence

doi:10.1128/AAC.00824-06

Scientific article

English

Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance

ContributorsPapon, Nicolas; Noël, Thierry; Florent, Martine; Gibot-Leclerc, Stéphanie; Jean, Dorothée; Chastin, Christiane; Villard, Jean; Chapeland-Leclerc, Florence

Published inAntimicrobial Agents and Chemotherapy, vol. 51, no. 1, p. 369-371

Publication date2007

Abstract

Inactivation of the FCY2 (cytosine permease), FCY1 (cytosine deaminase), and FUR1 (uracil phosphoribosyltransferase) genes in Candida lusitaniae produced two patterns of resistance to flucytosine. Mutant fur1 demonstrated resistance to 5-fluorouracil, whereas mutants fcy1 and fcy2 demonstrated fluconazole resistance in the presence of subinhibitory flucytosine concentrations.

Keywords

Alleles
Antifungal Agents/pharmacology
Candida/drug effects/enzymology/genetics
Cloning, Molecular
Cytosine Deaminase/genetics
DNA, Fungal/chemistry/genetics
Drug Resistance, Fungal/genetics
Fluconazole/pharmacology
Flucytosine/pharmacology
Fluorouracil/pharmacology
Fungal Proteins/genetics
Genetic Complementation Test
Microbial Sensitivity Tests
Molecular Sequence Data
Mutation
Pentosyltransferases/genetics
Plasmids/genetics
Sequence Analysis, DNA

Affiliation entities

Not a UNIGE publication

Research groups

Transplantation immunologie et osteoimmunologie (561)

Citation (ISO format)

PAPON, Nicolas et al. Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance. In: Antimicrobial Agents and Chemotherapy, 2007, vol. 51, n° 1, p. 369–371. doi: 10.1128/AAC.00824-06

Article (Published version)

Identifiers

PID : unige:149128
DOI : 10.1128/AAC.00824-06
PMID : 17060521

Additional URL for this publicationhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797687/

Journal ISSN0066-4804

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Molecular mechanism of flucytosine resistance in Candida lusitaniae: contribution of the FCY2, FCY1, and FUR1 genes to 5-fluorouracil and fluconazole cross-resistance

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