UNIGE document Scientific Article
previous document  unige:148412  next document
add to browser collection
Title

Transplanted embryonic neurons improve functional recovery by increasing activity in injured cortical circuits

Authors
Andreoli, Evgenia
show hidden authors show all authors [1 - 9]
Published in Cerebral Cortex. 2020, vol. 30, no. 8, p. 4708-4725
Abstract Transplantation of appropriate neuronal precursors after injury is a promising strategy to reconstruct cortical circuits, but the efficiency of these approaches remains limited. Here, we applied targeted apoptosis to selectively ablate layer II/III pyramidal neurons in the rat juvenile cerebral cortex and attempted to replace lost neurons with their appropriate embryonic precursors by transplantation. We demonstrate that grafted precursors do not migrate to replace lost neurons but form vascularized clusters establishing reciprocal synaptic contacts with host networks and show functional integration. These heterotopic neuronal clusters significantly enhance the activity of the host circuits without causing epileptic seizures and attenuate the apoptotic injury-induced functional deficits in electrophysiological and behavioral tests. Chemogenetic activation of grafted neurons further improved functional recovery, and the persistence of the graft was necessary for maintaining restored functions in adult animals. Thus, implanting neuronal precursors capable to form synaptically integrated neuronal clusters combined with activation-based approaches represents a useful strategy for helping long-term functional recovery following brain injury.
Keywords Cerebral cortexEmbryonic neural precursorsNeuronal apoptosisNeuronal circuitTransplantation
Identifiers
PMID: 32266929
Full text
Article (Published version) (2.5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Project
FNS: 31003A_140940/1
Citation
(ISO format)
ANDREOLI, Evgenia et al. Transplanted embryonic neurons improve functional recovery by increasing activity in injured cortical circuits. In: Cerebral Cortex, 2020, vol. 30, n° 8, p. 4708-4725. doi: 10.1093/cercor/bhaa075 https://archive-ouverte.unige.ch/unige:148412

90 hits

0 download

Update

Deposited on : 2021-02-01

Export document
Format :
Citation style :