Novel tissue engineered scaffolds are being developed in order to illustrate a more realistic model for studying cell behavior as well as functions and responses to external stimuli. Currently most of studies use two-dimensional (2D) monolayer cell cultures as an in vitro models (1). Although these models are reproductible, inexpensive and reliable they have some limitations when compared to what actually happens in vivo. Cells are embedded in an extracellular matrix (ECM), leading to the formation of cell-cell and cell-ECM junctions, thus conferring the tissue a three-dimensional (3D) structure which cannot be recapitulated with 2D standard cultures (2). In addition, cells are not isolated but interact with other cell types. For example, the sensatory capability of endothelial cells come from a combined activity of a large number of endothelial cells to sense flow and mechanical signals that lead to phenotypic and functional changes (3,4). Thus, 3D models are being more and more utilized to better represent these cellular interactions, as demonstrated through the development of more realistic cell culture models such as spheroids and organoids (5).