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Article scientifique
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Anglais

Neural priming of adipose-derived stem cells by cell-imprinted substrates

Publié dansBiofabrication
Date de publication2020
Résumé

Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical cues. We molded polydimethylsiloxane (PDMS) silicone substrates on fixed differentiated neural progenitor cells (ReNcellTM VM). The ReNcellTM cell line consists of immortalized human neural progenitor cells that are capable to differentiate into neural cells. The fabricated cell-imprinted silicone substrates represent the geometrical micro- and nanotopology of the target cell morphology. During the molding procedure, no transfer of cellular proteins was detectable. In the first test with undifferentiated ReNcellTM VM cells, the cell-imprinted substrates could accelerate neural differentiation. With adipose-derived stem cells cultivated on the imprinted substrates, we observed modifications of cell morphology, shifting from spread to elongated shape. Both immunofluorescence and quantitative gene expression analysis showed upregulation of neural stem cell and early neuronal markers. Our study, for the first time, demonstrated the effectiveness of cell-imprinted substrates for neural priming of adipose-derived stem cells for regenerative medicine applications.

Mots-clés
  • Neural tissue engineering
  • Cell imprinting
  • Human mesenchymal stem cells
  • Patterned substrate
  • Neural differentiation.
Citation (format ISO)
GHAZALI, Zahra Sadat et al. Neural priming of adipose-derived stem cells by cell-imprinted substrates. In: Biofabrication, 2020. doi: 10.1088/1758-5090/abc66f
Fichiers principaux (1)
Article (Accepted version)
Identifiants
ISSN du journal1758-5082
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Informations techniques

Création11/11/2020 17:22:00
Première validation11/11/2020 17:22:00
Heure de mise à jour15/03/2023 23:23:17
Changement de statut15/03/2023 23:23:16
Dernière indexation12/02/2024 13:20:56
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