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Scientific article
English

Structure and Inheritance of Sense and Anti-sense Transcripts from a Transposon in the Green Alga Chlamydomonas reinhardtii

Published inJournal of Molecular Biology, vol. 218, no. 2, p. 273-291
Publication date1991
Abstract

We have studied the transcription pattern of a 5700 base-pair transposon (TOC1) in Chlamydomonas reinhardtii. Northern blotting and nuclease S1 protection experiments define three classes of major TOC1 RNAs that accumulate to different levels in a number of strains and segregate independently in the progeny of crosses: class 1 RNAs are unstable near full-length sense transcripts whose 5' end maps to the left 217 base-pair repeat of TOC1, class 2 and class 3 RNAs are large, discrete chimaeric transcripts containing full-length sense (class 2) and anti-sense (class 3) copies of TOC1. Sequence motifs common to the 5' non-transcribed regions of C. reinhardtii genes were found upstream from the putative initiation site of class 1 transcripts. A functional polyadenylation site was located in the far-right 237 base-pair repeat of TOC1. Class 1 TOC1 transcripts are initiated, and probably terminated, within the terminal repeats of TOC1 and may represent retrotransposition intermediates. Class 2 and 3 TOC1 transcripts co-segregate with specific TOC1 elements identified on Southern blots. The loci that control the production of high levels of class 1 transcripts could correspond to specific TOC1 elements, i.e. only a few TOC1 elements are transcribed, or to a regulatory locus. The accumulation of an 11,500 to 12,000 base sense transcript (class 2) is reduced two- to fourfold by the presence of a 9500 to 9700 base antisense transcript (class 3). In contrast, the accumulation of the 5' ends of class 1 transcripts are unaffected by the anti-sense TOC1 transcript.

Funding
  • Swiss National Science Foundation - 3.328-086
Citation (ISO format)
DAY, Anil, ROCHAIX, Jean-David. Structure and Inheritance of Sense and Anti-sense Transcripts from a Transposon in the Green Alga <i>Chlamydomonas reinhardtii</i>. In: Journal of Molecular Biology, 1991, vol. 218, n° 2, p. 273–291. doi: 10.1016/0022-2836(91)90712-F
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ISSN of the journal0022-2836
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