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Decellularized human placenta supports hepatic tissue and allows rescue in acute liver failure

Kakabadze, Zurab
Kakabadze, Ann
Chakhunashvili, David
Karalashvili, Lia
Sharma, Yogeshwar
Gupta, Sanjeev
Published in Hepatology. 2018, vol. 67, no. 5, p. 1956-1969
Abstract Tissue engineering with scaffolds to form transplantable organs is of wide interest. Decellularized tissues have been tested for this purpose, although supplies of healthy donor tissues, vascular recellularization for perfusion, and tissue homeostasis in engineered organs pose challenges. We hypothesized that decellularized human placenta will be suitable for tissue engineering. The universal availability and unique structures of placenta for accommodating tissue, including presence of embedded vessels, were major attractions. We found decellularized placental vessels were reendothelialized by adjacent native cells and bridged vessel defects in rats. In addition, implantation of liver fragments containing all cell types successfully hepatized placenta with maintenance of albumin and urea synthesis, as well as hepatobiliary transport of 99m Tc-mebrofenin, up to 3 days in vitro. After hepatized placenta containing autologous liver was transplanted into sheep, tissue units were well-perfused and self-assembled. Histological examination indicated transplanted tissue retained hepatic cord structures with characteristic hepatic organelles, such as gap junctions, and hepatic sinusoids lined by endothelial cells, Kupffer cells, and other cell types. Hepatocytes in this neo-organ expressed albumin and contained glycogen. Moreover, transplantation of hepatized placenta containing autologous tissue rescued sheep in extended partial hepatectomy-induced acute liver failure. This rescue concerned amelioration of injury and induction of regeneration in native liver. The grafted hepatized placenta was intact with healthy tissue that neither proliferated nor was otherwise altered.
Keywords AnimalsFemaleHumansLiver/cytologyLiver Failure, Acute/therapyLiver Function TestsLiver Transplantation/methodsPlacenta/cytology/transplantationPregnancyRatsSheepTissue Engineering/methods
PMID: 29211918
Full text
Research group Création de tissus par bio-ingénieurie et régénération d'organes (1021)
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KAKABADZE, Zurab et al. Decellularized human placenta supports hepatic tissue and allows rescue in acute liver failure. In: Hepatology, 2018, vol. 67, n° 5, p. 1956-1969. doi: 10.1002/hep.29713

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Deposited on : 2020-07-10

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