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Title

Optimization of MS-Compatible Mobile Phases for IEX Separation of Monoclonal Antibodies

Authors
Farsang, Evelin
Horvath, Krisztian
Colas, Olivier
Beck, Alain
Published in LCGC North America. 2019, vol. 37, p. 34-38
Abstract Characterization of monoclonal antibodies (mAbs) and related products requires the identification of chromatographic peaks with mass spectrometry (MS). However, the conventional salt- and pH-gradient elution techniques used in ion-exchange chromatography (IEX) are inherently incompatible with MS. Ammonium acetate- and ammonium carbonate-based mobile phase systems have been recently applied in IEX-MS, but the influence of the eluent composition on peak shape and retention has not been discussed nor studied systematically, until now. The aim of the present study was to understand the impact of ionic strength, buffer capacity, and pH-response on the retention behavior and peak shape of mAb species.
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Structures
Research group Sciences analytiques
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FARSANG, Evelin et al. Optimization of MS-Compatible Mobile Phases for IEX Separation of Monoclonal Antibodies. In: LCGC North America, 2019, vol. 37, p. 34-38. https://archive-ouverte.unige.ch/unige:133139

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Deposited on : 2020-03-26

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