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Proteomics-based study of morphine effects on primary human brain microvascular endothelial cells

ContributorsReymond, Sandrineorcid
Master program titleMaster en Biologie
Defense date2020
Abstract

Morphine is a common antinociceptive drug, whose transport to the central nervous system (CNS) requires crossing the blood-brain barrier (BBB). However, its administration is associated with side effects reducing its efficiency, but the molecular processes are still not well understood. The present study evaluated the molecular mechanisms associated to morphine exposure on primary human brain microvascular endothelial cells (HBMECs) with a combination of mass spectrometry-based proteomics and pathway enrichment strategies. Pathway enrichment unveiled several affected pathways, including the NRF-2 pathway involved in oxidative stress response. We also underlined mitochondria dysfunctions, which could be related to oxidative stress. In addition, morphine ability to modulate HBMECs' secretion of TNF-α and IL-6 was observed, as well as an alteration of proteins expression involved in junctional complexes and cell adhesion. In conclusion, our study indicated morphine-induced modulations in redox homeostasis, immune response and barrier integrity in HBMECs, encouraging further investigation.

eng
Keywords
  • Morphine
  • Proteomics
  • Blood-Brain Barrier
Citation (ISO format)
REYMOND, Sandrine. Proteomics-based study of morphine effects on primary human brain microvascular endothelial cells. 2020.
Main files (1)
Master thesis
accessLevelRestricted
Secondary files (1)
Identifiers
  • PID : unige:130493
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Technical informations

Creation01/30/2020 10:33:00 AM
First validation01/30/2020 10:33:00 AM
Update time03/15/2023 9:07:49 PM
Status update03/15/2023 9:07:48 PM
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