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Scientific article
English

PSD-95 promotes synaptogenesis and multiinnervated spine formation through nitric oxide signaling

Contributors

Nikonenko, Iryna; Boda, Bernadett; Steen, Sylvain; Knott, Graham; Welker, Egbert; Muller, Dominique

Published in

The Journal of cell biology, vol. 183, no. 6, p. 1115-1127

Publication date

2008

Abstract

Postsynaptic density 95 (PSD-95) is an important regulator of synaptic structure and plasticity. However, its contribution to synapse formation and organization remains unclear. Using a combined electron microscopic, genetic, and pharmacological approach, we uncover a new mechanism through which PSD-95 regulates synaptogenesis. We find that PSD-95 overexpression affected spine morphology but also promoted the formation of multiinnervated spines (MISs) contacted by up to seven presynaptic terminals. The formation of multiple contacts was specifically prevented by deletion of the PDZ(2) domain of PSD-95, which interacts with nitric oxide (NO) synthase (NOS). Similarly, PSD-95 overexpression combined with small interfering RNA-mediated down-regulation or the pharmacological blockade of NOS prevented axon differentiation into varicosities and multisynapse formation. Conversely, treatment of hippocampal slices with an NO donor or cyclic guanosine monophosphate analogue induced MISs. NOS blockade also reduced spine and synapse density in developing hippocampal cultures. These results indicate that the postsynaptic site, through an NOS-PSD-95 interaction and NO signaling, promotes synapse formation with nearby axons.

Keywords

  • Animals
  • Cyclic GMP/analogs & derivatives/pharmacology
  • Dendritic Spines/drug effects/enzymology/metabolism/ultrastructure
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Membrane Proteins/metabolism
  • Mice
  • NIH 3T3 Cells
  • Nitric Oxide/metabolism
  • Nitric Oxide Synthase Type I/metabolism
  • Nitroso Compounds/pharmacology
  • Organogenesis/drug effects
  • Protein Binding/drug effects
  • Pyramidal Cells/drug effects/enzymology/ultrastructure
  • Rats
  • Signal Transduction/drug effects
  • Synapses/drug effects/enzymology/metabolism/ultrastructure
  • Transfection

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

NIKONENKO, Iryna et al. PSD-95 promotes synaptogenesis and multiinnervated spine formation through nitric oxide signaling. In: The Journal of cell biology, 2008, vol. 183, n° 6, p. 1115–1127. doi: 10.1083/jcb.200805132

Main files (1)

Article (Accepted version)

Secondary files (1)

Supplemental data

Identifiers

Additional URL for this publication

http://jcb.rupress.org/cgi/content/full/183/6/1115

Journal ISSN

0021-9525
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Technical informations

Creation

01/04/2009 15:15:00

First validation

01/04/2009 15:15:00

Update

14/03/2023 15:03:58

Status update

14/03/2023 15:03:58

Last indexation

29/10/2024 11:25:33
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