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Title

CRISPR/cas9-mediated generation of tetracycline repressor-based inducible knockdown in toxoplasma gondii

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Published in Tonkin C.J. Toxoplasma gondii : methods and protocols. New York: Humana Press. 2020, p. 125-141
Collection Methods in molecular biology; 2071
Abstract The phylum Apicomplexa groups numerous pathogenic protozoan parasites including Plasmodium, the causative agent of malaria, Cryptosporidium which can cause severe gastrointestinal infections, as well as Babesia, Eimeria, and Theileria that account for considerable economic burdens to poultry and cattle industry. Toxoplasma gondii is the most ubiquitous and opportunistic member of this phylum able to infect all warm-blooded animals and responsible for severe disease in immunocompromised individuals and unborn fetuses.Due to its ease of cultivation and genetic tractability T. gondii has served as recipient for the transfer and adaptation of multiple genetic tools developed to control gene expression. In these parasites, a collection of tight conditional systems exists to control gene expression at the levels of transcription, RNA degradation or protein stability. The recent implementation of the CRISPR/Cas9 technology considerably reduces time and effort to generate transgenic parasites and at the same time increases to an ultimate level of precision the editing of the parasite genome. Here, we provide a step-by-step protocol for CRISPR/Cas9-mediated generation of tetracycline repressor-based inducible knockdown in T. gondii.
Identifiers
PMID: 31758450
ISBN: 978-1-4939-9856-2
Full text
Book chapter (Published version) (732 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Biologie d'un parasite intracellulaire obligatoire (773)
Project FNS: 310030B_166678
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(ISO format)
JACOT, Damien, SOLDATI-FAVRE, Dominique. CRISPR/cas9-mediated generation of tetracycline repressor-based inducible knockdown in toxoplasma gondii. In: Tonkin C.J. (Ed.). Toxoplasma gondii : methods and protocols. New York : Humana Press, 2020. p. 125-141. (Methods in molecular biology; 2071) doi: 10.1007/978-1-4939-9857-9_7 https://archive-ouverte.unige.ch/unige:126988

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Deposited on : 2019-11-26

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