Scientific article
Open access

Identification of new DNA-associated proteins from Waddlia chondrophila

Published inScientific Reports, vol. 9, no. 1, 4885
Publication date2019

Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ66), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.

  • Swiss National Science Foundation - Sinergia CRSII3-141837
Citation (ISO format)
BARSY, Marie de et al. Identification of new DNA-associated proteins from Waddlia chondrophila. In: Scientific Reports, 2019, vol. 9, n° 1, p. 4885. doi: 10.1038/s41598-019-40732-1
Main files (1)
Article (Published version)
ISSN of the journal2045-2322

Technical informations

Creation10/12/2019 9:53:00 AM
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