Article (Published version) (2.5 MB) - 
Free access
Highlights
Scientific Article
unige:124469 
 |
Identification of new DNA-associated proteins from Waddlia chondrophila |
|
| Authors | ||
| Published in | Scientific Reports. 2019, vol. 9, no. 1, p. 4885 | |
| Abstract | Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ66), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain. | |
| Identifiers | PMID: 30894592 | |
| Full text | ||
| Structures | ||
| Research group | Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895) | |
| Project | FNS: Sinergia CRSII3-141837 | |
| Citation (ISO format) | BARSY, Marie de et al. Identification of new DNA-associated proteins from Waddlia chondrophila. In: Scientific Reports, 2019, vol. 9, n° 1, p. 4885. doi: 10.1038/s41598-019-40732-1 https://archive-ouverte.unige.ch/unige:124469 |
154 hits 
68 downloads 
Contact an author
Update