Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA.

Delaby, Marie; Panis, Gaël; Viollier, Patrick

doi:10.1093/nar/gkz846

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Title		Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA.
Authors		Delaby, Marie Panis, Gaël Viollier, Patrick
Published in		Nucleic acids research. 2019
Abstract		Many bacteria acquire dissemination and virulence traits in G1-phase. CtrA, an essential and conserved cell cycle transcriptional regulator identified in the dimorphic alpha-proteobacterium Caulobacter crescentus, first activates promoters in late S-phase and then mysteriously switches to different target promoters in G1-phase. We uncovered a highly conserved determinant in the DNA-binding domain (DBD) of CtrA uncoupling this promoter switch. We also show that it reprograms CtrA occupancy in stationary cells inducing a (p)ppGpp alarmone signal perceived by the RNA polymerase beta subunit. A simple side chain modification in a critical residue within the core DBD imposes opposing developmental phenotypes and transcriptional activities of CtrA and a proximal residue can direct CtrA towards activation of the dispersal (G1-phase) program. Hence, we propose that this conserved determinant in the CtrA primary structure dictates promoter reprogramming during the growth transition in other alpha-proteobacteria that differentiate from replicative cells into dispersal cells.
Identifiers		DOI: 10.1093/nar/gkz846 PMID: 31598724
Full text		Article (Published version) (2.8 MB) - Free access
Structures		Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group		Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)
Project		Swiss National Science Foundation: 31003A 182576
Citation (ISO format)		DELABY, Marie, PANIS, Gaël, VIOLLIER, Patrick. Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA. In: Nucleic Acids Research, 2019. doi: 10.1093/nar/gkz846 https://archive-ouverte.unige.ch/unige:124465