Article (Published version) (2.8 MB) - Free access
Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA.
|Published in||Nucleic acids research. 2019|
|Abstract||Many bacteria acquire dissemination and virulence traits in G1-phase. CtrA, an essential and conserved cell cycle transcriptional regulator identified in the dimorphic alpha-proteobacterium Caulobacter crescentus, first activates promoters in late S-phase and then mysteriously switches to different target promoters in G1-phase. We uncovered a highly conserved determinant in the DNA-binding domain (DBD) of CtrA uncoupling this promoter switch. We also show that it reprograms CtrA occupancy in stationary cells inducing a (p)ppGpp alarmone signal perceived by the RNA polymerase beta subunit. A simple side chain modification in a critical residue within the core DBD imposes opposing developmental phenotypes and transcriptional activities of CtrA and a proximal residue can direct CtrA towards activation of the dispersal (G1-phase) program. Hence, we propose that this conserved determinant in the CtrA primary structure dictates promoter reprogramming during the growth transition in other alpha-proteobacteria that differentiate from replicative cells into dispersal cells.|
|Research group||Régulation du cycle cellulaire dans C.crescentus, une bactérie asymétrique (895)|
Swiss National Science Foundation: 31003A 182576
|DELABY, Marie, PANIS, Gaël, VIOLLIER, Patrick. Bacterial cell cycle and growth phase switch by the essential transcriptional regulator CtrA. In: Nucleic Acids Research, 2019. doi: 10.1093/nar/gkz846 https://archive-ouverte.unige.ch/unige:124465|