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Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin

Stoeck, Ina Karen
Hänni, Christine
Bleck, Christopher Karl Ernst
Balistreri, Giuseppe
Helenius, Ari
Published in EMBO journal. 2012, vol. 31, no. 10, p. 2350-2364
Abstract Caveolae are specialized domains present in the plasma membrane (PM) of most mammalian cell types. They function in signalling, membrane regulation, and endocytosis. We found that the Eps-15 homology domain-containing protein 2 (EHD2, an ATPase) associated with the static population of PM caveolae. Recruitment to the PM involved ATP binding, interaction with anionic lipids, and oligomerization into large complexes (60-75S) via interaction of the EH domains with intrinsic NPF/KPF motifs. Hydrolysis of ATP was essential for binding of EHD2 complexes to caveolae. EHD2 was found to undergo dynamic exchange at caveolae, a process that depended on a functional ATPase cycle. Depletion of EHD2 by siRNA or expression of a dominant-negative mutant dramatically increased the fraction of mobile caveolar vesicles coming from the PM. Overexpression of EHD2, in turn, caused confinement of cholera toxin B in caveolae. The confining role of EHD2 relied on its capacity to link caveolae to actin filaments. Thus, EHD2 likely plays a key role in adjusting the balance between PM functions of stationary caveolae and the role of caveolae as vesicular carriers.
Keywords Actins/metabolismAdenosine Triphosphate/metabolismCarrier Proteins/genetics/metabolismCaveolae/metabolismCell Membrane/metabolismGene DeletionGene ExpressionGene SilencingHeLa CellsHumansProtein BindingProtein Interaction Domains and Motifs
PMID: 22505029
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Article (Published version) (3 MB) - public document Free access
Research group Contrôle subcellulaire des récepteurs de signalisation (1005)
Autre: Boehringer Ingelheim Fonds PhD fellowship
(ISO format)
STOEBER, Miriam Carolin et al. Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin. In: EMBO Journal, 2012, vol. 31, n° 10, p. 2350-2364. doi: 10.1038/emboj.2012.98

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Deposited on : 2019-09-12

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