Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs

Lau, Kelvin Ka Ching; Podolec, Roman; Chappuis, Richard; Ulm, Roman; Hothorn, Michael

doi:10.15252/embj.2019102140

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Title		Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs
Authors		Lau, Kelvin Ka Ching Podolec, Roman Chappuis, Richard Ulm, Roman Hothorn, Michael
Published in		EMBO Journal. 2019, p. e102140
Abstract		Plants sense different parts of the sun's light spectrum using distinct photoreceptors, which signal through the E3 ubiquitin ligase COP1. Here, we analyze why many COP1-interacting transcription factors and photoreceptors harbor sequence-divergent Val-Pro (VP) motifs that bind COP1 with different binding affinities. Crystal structures of the VP motifs of the UV-B photoreceptor UVR8 and the transcription factor HY5 in complex with COP1, quantitative binding assays, and reverse genetic experiments together suggest that UVR8 and HY5 compete for COP1. Photoactivation of UVR8 leads to high-affinity cooperative binding of its VP motif and its photosensing core to COP1, preventing COP1 binding to its substrate HY5. UVR8-VP motif chimeras suggest that UV-B signaling specificity resides in the UVR8 photoreceptor core. Different COP1-VP peptide motif complexes highlight sequence fingerprints required for COP1 targeting. The blue-light photoreceptors CRY1 and CRY2 also compete with transcription factors for COP1 binding using similar VP motifs. Thus, our work reveals that different photoreceptors and their signaling components compete for COP1 via a conserved mechanism to control different light signaling cascades.
Identifiers		DOI: 10.15252/embj.2019102140 PMID: 31304983
Full text		Article (Published version) (2.5 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département de botanique et biologie végétale
Research groups		Groupe Hothorn Groupe Ulm
Projects		FNS: 31003A_175774 FP7: UV-B PERCEPTION
Citation (ISO format)		LAU, Kelvin Ka Ching et al. Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs. In: EMBO Journal, 2019, p. e102140. doi: 10.15252/embj.2019102140 https://archive-ouverte.unige.ch/unige:121405