UNIGE document Doctoral Thesis
previous document  unige:120317  next document
add to browser collection
Title

DNA-Protein Crosslinks Formation and Processing in Saccharomyces cerevisiae

Author
Director
Defense Thèse de doctorat : Univ. Genève, 2019 - Sc. Vie 20 - 2019/05/31
Abstract Several environmental agents and intracellular metabolites promote formation of a covalent bond between protein and DNA, thus stabilising one of the most toxic DNA lesions – a DNA-protein crosslink (DPC). Bulky in their nature, DPCs prevent genuine progression of the replication and transcription machineries, thus seriously threatening DNA integrity. This work reveals two novel pathways contributing to cell survival in the presence of toxic DPC lesions: the SUMO posttranslational modification and the aspartic protease Ddi1. SUMOylation is described here to regulate the outcomes of toxic Top1-DNA trapping or formation of chemically identical covalent DPC lesions. Ddi1 is required for the efficient elimination Top1-DNA and other DPC adducts in a protease dependent manner, and is therefore proposed to act as a novel dedicated proteolytic enzyme contributing to DPC disassembly. In summary, the manuscript presents novel insights into the molecular mechanisms underlying the cellular response to toxic DPC lesions.
Keywords DNA-protein crosslinkDPCYeastDNA repairSUMOUbiquitinUlp1Siz2Ddi1Wss1ProteaseTop1Flp recombinase26S proteasomeRpb1
Identifiers
URN: urn:nbn:ch:unige-1203172
Full text
Thesis (12 MB) - private document Private access (until 2020-07-02)
Structures
Citation
(ISO format)
SERBYN, Nataliia. DNA-Protein Crosslinks Formation and Processing in Saccharomyces cerevisiae. Université de Genève. Thèse, 2019. https://archive-ouverte.unige.ch/unige:120317

32 hits

1 download

Update

Deposited on : 2019-07-03

Export document
Format :
Citation style :