en
Doctoral thesis
English

Diversity in protein post-translational modifications and their critical impacts on the lytic cycle of Toxoplasma gondii

ContributorsDogga, Sunil Kumar
Defense date2018-02-15
Abstract

Apicomplexan phylum comprises obligate intracellular parasites, infecting a wide range of hosts from vertebrates to invertebrates. My thesis covers two main aspects of their biology. Firstly, I delineated the establishment of intracellular parasitism in Apicomplexans over the course of evolution by studying the phylogeny, morphology, metabolic and invasive capabilities of a fish coccidian parasite, Goussia janae. In the second section of the thesis, I dealt with the investigation of the role of two post-translational modifications, proteolytic processing and palmitoylation, of proteins. We identified and characterized the essentiality of an aspartyl protease 3 (TgASP3) for the lytic cycle of the parasite by highlighting its role as a maturase for integral secretory organelle proteins. Furthermore, we identified a potential drug candidate 49c, a peptidomimetic inhibitor that targets. We characterized the essential nature of 2 inner membrane complex (IMC)-resident palmitoyl acyl transferases, TgDHHC2 and TgDHHC14, and a Golgi-resident TgDHHC5.

eng
Citation (ISO format)
DOGGA, Sunil Kumar. Diversity in protein post-translational modifications and their critical impacts on the lytic cycle of Toxoplasma gondii. 2018. doi: 10.13097/archive-ouverte/unige:113906
Main files (1)
Thesis
accessLevelRestricted
Identifiers
519views
10downloads

Technical informations

Creation02/05/2019 9:38:00 PM
First validation02/05/2019 9:38:00 PM
Update time03/15/2023 3:40:41 PM
Status update03/15/2023 3:40:40 PM
Last indexation05/03/2024 7:07:07 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack