Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

Izui, Shozo; Lambert, Paul Henri; Miescher, P. A.

Scientific article

English

Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

ContributorsIzui, Shozo; Lambert, Paul Henri; Miescher, P. A.

Published inClinical and experimental immunology, vol. 26, no. 3, p. 425-430

Publication date1976

Abstract

A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.

Keywords

Antibodies/ analysis
Binding Sites, Antibody
DNA/ immunology
Humans
Immunologic Techniques
Leukemia/immunology
Lupus Erythematosus, Systemic/immunology
Protein Binding/drug effects
Sodium Dodecyl Sulfate/pharmacology

Affiliation entities

Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

Citation (ISO format)

IZUI, Shozo, LAMBERT, Paul Henri, MIESCHER, P. A. Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent. In: Clinical and experimental immunology, 1976, vol. 26, n° 3, p. 425–430.

Article

Identifiers

PID : unige:11338
PMID : 795575

Commercial URLhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1540975/pdf/clinexpimmunol00244-0053.pdf

ISSN of the journal0009-9104

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Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

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