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Scientific article
English

Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent

Published inClinical and experimental immunology, vol. 26, no. 3, p. 425-430
Publication date1976
Abstract

A modified 125I-labelled DNA-binding test was developed. By using an anionic detergent, 0-025% sodium dodecyl sulphate, the non-specific binding of DNA by basic proteins was completely eliminated, while the specific binding of DNA by anti-DNA antibodies was not interfered with. The level of DNA-binding activity in normal human sera was decreased in the modified DNA-binding test, while its sensitivity was similar to that of the Farr assay in presence of sera from SLE patients.

Keywords
  • Antibodies/ analysis
  • Binding Sites, Antibody
  • DNA/ immunology
  • Humans
  • Immunologic Techniques
  • Leukemia/immunology
  • Lupus Erythematosus, Systemic/immunology
  • Protein Binding/drug effects
  • Sodium Dodecyl Sulfate/pharmacology
Citation (ISO format)
IZUI, Shozo, LAMBERT, Paul Henri, MIESCHER, P. A. Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent. In: Clinical and experimental immunology, 1976, vol. 26, n° 3, p. 425–430.
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Article
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Identifiers
  • PID : unige:11338
  • PMID : 795575
ISSN of the journal0009-9104
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