en
Scientific article
Open access
English

The N-terminal Ac-EEED sequence plays a role in alpha-smooth-muscle actin incorporation into stress fibers

Published inJournal of cell science, vol. 118, no. Pt 7, p. 1395-1404
Publication date2005
Abstract

We have previously shown that the N-terminal sequence AcEEED of alpha-smooth-muscle actin causes the loss of alpha-smooth-muscle actin from stress fibers and a decrease in cell contractility when introduced in myofibroblasts as a cell-penetrating fusion peptide. Here, we have investigated the function of this sequence on stress fiber organization in living cells, using enhanced green fluorescent protein (EGFP)-tagged alpha-smooth-muscle actin. The fusion peptide provokes the gradual disappearance of EGFP fluorescence of alpha-smooth-muscle actin from stress fibers and the formation of hitherto unknown rod-like structures. In addition to alpha-smooth-muscle actin, these structures contain cytoplasmic actins, gelsolin and cofilin but not other major actin-binding proteins. These rod-like structures are also visible in wild-type fibroblasts during normal cell spreading, suggesting that they represent a physiological step in the organization of alpha-smooth-muscle actin in stress fibers. Fluorescence-recovery-after-photobleaching experiments suggest that the fusion peptide reduces the dynamics of alpha-smooth-muscle actin and its incorporation in stress fibers. Here, we propose a new mechanism of how alpha-smooth-muscle actin is incorporated in stress fibers involving the sequence Ac-EEED.

Keywords
  • Actins/drug effects/ physiology/ultrastructure
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Movement/physiology
  • Fibroblasts/drug effects/metabolism/ultrastructure
  • Fluorescence Recovery After Photobleaching
  • Green Fluorescent Proteins/genetics
  • Molecular Sequence Data
  • Oligopeptides/pharmacology/ physiology
  • Peptide Fragments/pharmacology
  • Protein Binding
  • Rats
  • Recombinant Fusion Proteins/genetics/metabolism
  • Stress Fibers/drug effects/ physiology/ultrastructure
  • Time Factors
Citation (ISO format)
CLEMENT, Sophie et al. The N-terminal Ac-EEED sequence plays a role in alpha-smooth-muscle actin incorporation into stress fibers. In: Journal of cell science, 2005, vol. 118, n° Pt 7, p. 1395–1404. doi: 10.1242/jcs.01732
Main files (1)
Article
accessLevelPublic
Identifiers
ISSN of the journal0021-9533
536views
143downloads

Technical informations

Creation08/27/2010 1:34:14 PM
First validation08/27/2010 1:34:14 PM
Update time03/14/2023 4:02:57 PM
Status update03/14/2023 4:02:57 PM
Last indexation02/12/2024 7:15:55 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack