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Scientific article
English

Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice

Published inThe Journal of immunology, vol. 135, no. 6, p. 3704-3711
Publication date1985
Abstract

Mice bearing the recessive gene lpr develop an autoimmune syndrome associated with a massive lymphadenopathy, both of which are age and thymus dependent. The predominant accumulating cells in lymphoid tissue of lpr/lpr mice are Thy-1+ but express neither of the mature T cell markers, Lyt-2 or L3T4. We have purified this Lyt-2-/L3T4- subset and examined its phenotype. These cells are not actively cycling, do not express interleukin-2 (IL 2) receptors nor significant levels of antigen receptor, but do express the B cell marker B220. In vitro growth conditions were examined for the lpr Lyt-2-/L3T4- subset. By using a combination of phorbol ester and IL 2, these cells acquired transient expression of IL 2 receptors and grew in an IL 2-dependent manner. Furthermore, these proliferating cells underwent differentiation to a more mature T cell phenotype, with loss of cell surface B220 and acquisition, by a portion, of antigen receptor and Lyt-2. The possible parallels with normal T cell maturation are discussed.

Keywords
  • Animals
  • Antigens, Ly/genetics
  • Antigens, Surface/genetics
  • Cell Cycle/drug effects
  • Cell Differentiation/drug effects
  • Concanavalin A/pharmacology
  • Lymph Nodes/cytology
  • Lymphocyte Activation/drug effects
  • Lymphocyte Function-Associated Antigen-1
  • Mice
  • Mice, Inbred C57BL/ genetics
  • Phenotype
  • Receptors, Immunologic/metabolism
  • Receptors, Interleukin-2
  • T-Lymphocytes, Cytotoxic/ classification/cytology/immunology/metabolism
  • Tetradecanoylphorbol Acetate/pharmacology
Affiliation Not a UNIGE publication
Citation (ISO format)
BUDD, R. C. et al. Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice. In: The Journal of immunology, 1985, vol. 135, n° 6, p. 3704–3711.
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ISSN of the journal0022-1767
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