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Influence of Gilbert's syndrome on the formation of ethyl glucuronide

Huppertz, Laura M
Gunsilius, Leonie
Weinmann, Wolfgang
Thierauf-Emberger, Annette
Published in International Journal of Legal Medicine. 2015, vol. 129, no. 5, p. 1005-1010
Abstract A drinking experiment with participants suffering from Gilbert's syndrome was performed to study the possible influence of this glucuronidation disorder on the formation of ethyl glucuronide (EtG). Gilbert's syndrome is a rather common and, in most cases, asymptomatic congenital metabolic aberration with a prevalence of about 5 %. It is characterized by a reduction of the enzyme activity of the uridine diphosphate glucuronosyltransferase (UGT) isoform 1A1 up to 80 %. One of the glucuronidation products is EtG, which is formed in the organism following exposure to ethanol. EtG is used as a short-term marker for ethyl alcohol consumption to prove abstinence in various settings. After 2 days of abstinence from ethanol and giving a void urine sample, 30 study participants drank 0.1 L of sparkling wine (9 g ethanol). 3, 6, 12, and 24 h after drinking, urine samples were collected. 3 hours after drinking, an additional blood sample was taken, in which liver enzyme activities, ethanol, hematological parameters, and bilirubin were measured. EtG and ethyl sulfate (EtS), another short-term marker of ethanol consumption, were determined in the urine samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS); creatinine was measured photometrically. In all participants, EtG and EtS were detected in concentrations showing a wide range (EtG: 3 h sample 0.5-18.43 mg/L and 6 h sample 0.67-13.8 mg/L; EtS: 3 h sample 0.87-6.87 mg/L and 6 h sample 0.29-4.48 mg/L). No evidence of impaired EtG formation was found. Thus, EtG seems to be a suitable marker for ethanol consumption even in individuals with Gilbert's syndrome.
Keywords AdolescentAdultAgedAlcohol DrinkingBiomarkers/urineCentral Nervous System Depressants/blood/pharmacokineticsChromatography, LiquidEthanol/blood/pharmacokineticsFemaleGilbert Disease/metabolismGlucuronates/urineHumansMaleMiddle AgedSulfuric Acid Esters/urineTandem Mass SpectrometryYoung Adult
PMID: 25680552
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HUPPERTZ, Laura M et al. Influence of Gilbert's syndrome on the formation of ethyl glucuronide. In: International Journal of Legal Medicine, 2015, vol. 129, n° 5, p. 1005-1010. https://archive-ouverte.unige.ch/unige:111142

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Deposited on : 2018-11-21

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