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Title

E-selectin binding promotes neutrophil activation in vivo in E-selectin transgenic mice
Authors
Araki, Masatake
Araki, Kimi
Miyazaki, Y.
Iwamoto, Masahiro
Izui, Shozo
Yamamura, K.
Vassalli, Pierre
Published in Biochemical and Biophysical Research Communications. 1996, vol. 224, no. 3, p. 825-830
Abstract E-selectin is a membrane protein expressed by endothelial cells activated by cytokines released during the inflammatory process; it plays an important role in neutrophil emigration into inflamed tissues. To further explore in vivo the function of E-selectin, we have generated transgenic mouse line expressing E-selectin under the control of a chicken beta-actin promoter. In these mice, the number of blood neutrophils was reduced, without any other obvious phenotype or tissue damage. These neutrophils, however, displayed two significant changes: first, an alteration in the levels of expression of two membrane receptors involved in neutrophil adhesion to endothelial cells, namely a marked increased in the Mac-1 antigen (CD11b/CD18) and a decrease in the Mel-14 antigen (L-selectin); second, an increased oxidative activity when compared to blood neutrophils of non-transgenic mice, as shown by their capacity to oxidize 2',7'-dichlorofluorescein (DCFH) into a fluorescent compound. These observations indicate that the binding of E-selection with neutrophils bearing its ligands promotes neutrophil activation in vivo.
Keywords Actins/genetics — Animals — Base Sequence — Bone Marrow/metabolism — Bone Marrow Cells — E-Selectin/genetics/ metabolism — Fluorescence — Liver/metabolism — Lymphocyte Activation — Mice — Mice, Transgenic — Molecular Sequence Data — Muscles/metabolism — Myocardium/metabolism — Neutrophil Activation — Neutrophils/ immunology/metabolism — Promoter Regions, Genetic — Protein Binding — RNA, Messenger/genetics
Identifiers
PMID: 8713130
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Structures
Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Citation
(ISO format) 		ARAKI, Masatake et al. E-selectin binding promotes neutrophil activation in vivo in E-selectin transgenic mice. In: Biochemical and Biophysical Research Communications, 1996, vol. 224, n° 3, p. 825-830. https://archive-ouverte.unige.ch/unige:11114

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Deposited on : 2010-08-27

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