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Title

Deficiency in IL-1 Receptor Type 2 Aggravates K/BxN Serum Transfer-Induced Arthritis in Mice but Has No Impact on Systemic Inflammatory Responses

Authors
Martin, Praxedis Sina
Published in Journal of Immunology. 2017, vol. 198, no. 7, p. 2916-2926
Abstract The biological activity of IL-1 is tightly regulated by the specific receptor antagonist (IL-1Ra) and the decoy receptor IL-1 receptor type 2 (IL-1R2). The role of IL-1Ra has been well demonstrated in IL-1Ra-deficient mice. In contrast, the role of endogenous IL-1R2 remains widely unknown. To define the functional role of endogenous IL-1R2 in the K/BxN serum transfer arthritis model and in IL-1β- or LPS-induced systemic inflammation in vivo, IL-1R2-/- mice were created and compared with wild type mice. IL-1R2-/- mice bred habitually and exhibited a normal phenotype. IL-1R2 deficiency aggravated arthritis severity and increased mRNA levels for key cytokines and chemokines such as IL-6, IL-1β, Cxcl-1, and Cxcl-2 significantly in ankles. There was no effect of IL-1R2 deficiency on the cell-autonomous cytokine response to IL-1β in the tested cell types, i.e., neutrophils, macrophages, and fibroblasts, but IL-1R2 deficiency on neutrophils increased the IL-1-induced response of fibroblasts in trans. Furthermore, IL-1β induced shedding of IL-1R2 in vivo. Inflammatory responses to IL-1β and LPS-induced mortality were not different in IL-1R2-/- compared with wild type mice. Our data demonstrate that the decoy receptor IL-1R2 plays an important inhibitory role in local IL-1- and neutrophil-dependent tissue inflammation as shown in the K/BxN serum transfer arthritis model. In contrast to IL-1Ra, IL-1R2 appears to be less crucial for systemic responses to acute administration of IL-1 or LPS.
Keywords AnimalsArthritis, Experimental/immunologyImmunohistochemistryInflammation/immunologyMiceMice, KnockoutReal-Time Polymerase Chain ReactionReceptors, Interleukin-1 Type II/immunology
Identifiers
PMID: 28235865
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Article (Published version) (1.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Mécanisme de l'inflammation articulaire (44)
Project FNS: 310030_152638
Citation
(ISO format)
MARTIN, Praxedis Sina et al. Deficiency in IL-1 Receptor Type 2 Aggravates K/BxN Serum Transfer-Induced Arthritis in Mice but Has No Impact on Systemic Inflammatory Responses. In: Journal of Immunology, 2017, vol. 198, n° 7, p. 2916-2926. https://archive-ouverte.unige.ch/unige:110653

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Deposited on : 2018-11-12

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