UNIGE document Scientific Article
previous document  unige:108943  next document
add to browser collection

Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells

Slenders, Eli
Bové, Hannelore
Urbain, Mathias
Mugnier, Yannick
Sonay, Ali Yasin
Pantazis, Periklis
Vanden Berghe, Pieter
show hidden authors show all authors [1 - 10]
Published in Journal of Physical Chemistry Letters. 2018, vol. 9, p. 6112-6118
Abstract The absence of photobleaching, blinking, and saturation combined with a high contrast provides unique advantages of higher-harmonic generating nanoparticles over fluorescent probes, allowing for prolonged correlation spectroscopy studies. We apply the coherent intensity fluctuation model to study the mobility of second harmonic generating nanoparticles. A concise protocol is presented for quantifying the diffusion coefficient from a single spectroscopy measurement without the need for separate point-spread-function calibrations. The technique's applicability is illustrated on nominally 56 nm LiNbO3 nanoparticles. We perform label-free raster image correlation spectroscopy imaging in aqueous suspension and spatiotemporal image correlation spectroscopy in A549 human lung carcinoma cells. In good agreement with the expected theoretical result, the measured diffusion coefficient in water at room temperature is (7.5 ± 0.3) µm^2/s. The diffusion coefficient in the cells is more than 10^3 times lower, and heterogeneous, with an average of (3.7 ± 1.5) x 10^(-3) µm^2/s.
Keywords Nonlinear opticsCorrelation spectroscopyNanoparticlesHarmonic generation
Full text
Research group Groupe Wolf
(ISO format)
SLENDERS, Eli et al. Image Correlation Spectroscopy with Second Harmonic Generating Nanoparticles in Suspension and in Cells. In: Journal of Physical Chemistry Letters, 2018, vol. 9, p. 6112-6118. https://archive-ouverte.unige.ch/unige:108943

7 hits



Deposited on : 2018-10-09

Export document
Format :
Citation style :