Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside

Waridel, Patrice; Wolfender, Jean-Luc; Lachavanne, Jean-Bernard; Hostettmann, Kurt

doi:10.1016/j.phytochem.2004.06.031

Scientific article

English

Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside

ContributorsWaridel, Patrice; Wolfender, Jean-Luc; Lachavanne, Jean-Bernard; Hostettmann, Kurt

Published inPhytochemistry, vol. 65, no. 16, p. 2401-2410

Publication date2004

Abstract

The polar extracts of Potamogeton pectinatus, P. lucens, P. perfoliatus and P. crispus (Potamogetonaceae) were analyzed by HPLC-UV-MS and their chromatographic profiles were very similar. The polar constituents of P. pectinatus were more exhaustively investigated by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, which allowed the on-line identification of various known flavones (dereplication). One of these compounds, luteolin 3'-O-glucoside, has never been characterized in the Potamogeton genus. The HPLC-UV-MS and HPLC-NMR analyses revealed also the presence of ent-labdane diterpene glycosides in the polar extracts of P. pectinatus and P. lucens and led to the isolation of a new ent-labdane diglycoside from P. pectinatus, beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-15,16-epoxy-12-oxo-8(17),13(16),14-ent-labdatrien-19-oate. (C) 2004 Elsevier Ltd. All rights reserved.

Keywords

Potamogeton pectinatus
P. lucens
P. perfoliatus
P. crispus
Potamogetonaceae
Spectroscopic analysis
HPLC-MS
HPLC-NMR
Ent-Labdane diterpenes
Flavonoids

Affiliation entities

Citation (ISO format)

WARIDEL, Patrice et al. Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside. In: Phytochemistry, 2004, vol. 65, n° 16, p. 2401–2410. doi: 10.1016/j.phytochem.2004.06.031

Article (Published version)

Identifiers

PID : unige:10840
DOI : 10.1016/j.phytochem.2004.06.031

Additional URL for this publicationhttp://linkinghub.elsevier.com/retrieve/pii/S0031942204002936

Journal ISSN0031-9422

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Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside

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