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Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside

Published inPhytochemistry, vol. 65, no. 16, p. 2401-2410
Publication date2004
Abstract

The polar extracts of Potamogeton pectinatus, P. lucens, P. perfoliatus and P. crispus (Potamogetonaceae) were analyzed by HPLC-UV-MS and their chromatographic profiles were very similar. The polar constituents of P. pectinatus were more exhaustively investigated by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, which allowed the on-line identification of various known flavones (dereplication). One of these compounds, luteolin 3'-O-glucoside, has never been characterized in the Potamogeton genus. The HPLC-UV-MS and HPLC-NMR analyses revealed also the presence of ent-labdane diterpene glycosides in the polar extracts of P. pectinatus and P. lucens and led to the isolation of a new ent-labdane diglycoside from P. pectinatus, beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-15,16-epoxy-12-oxo-8(17),13(16),14-ent-labdatrien-19-oate. (C) 2004 Elsevier Ltd. All rights reserved.

Keywords
  • Potamogeton pectinatus
  • P. lucens
  • P. perfoliatus
  • P. crispus
  • Potamogetonaceae
  • Spectroscopic analysis
  • HPLC-MS
  • HPLC-NMR
  • Ent-Labdane diterpenes
  • Flavonoids
Citation (ISO format)
WARIDEL, Patrice et al. Identification of the polar constituents of Potamogeton species by HPLC-UV with post-column derivatization, HPLC-MSn and HPLC-NMR, and isolation of a new ent-labdane diglycoside. In: Phytochemistry, 2004, vol. 65, n° 16, p. 2401–2410. doi: 10.1016/j.phytochem.2004.06.031
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ISSN of the journal0031-9422
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