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Toxoplasma gondii TFP1 is an essential transporter family protein critical for microneme maturation and exocytosis

Published in Molecular Microbiology. 2018
Abstract Invasion and egress are two key steps in the lytic cycle of Apicomplexa that are governed by the sequential discharge of proteins from two apical secretory organelles called micronemes and rhoptries. In Toxoplasma gondii, the biogenesis of these specialized organelles depends on the post Golgi trafficking machinery, forming an endosomal-like compartment (ELC) resembling endomembrane systems found in eukaryotes. In this study, we have characterized four phylogenetically related Transporter Facilitator Proteins (TFPs) conserved among the apicomplexans. TFP1 localises to the micronemes and ELC, TFP2 and TFP3 to the rhoptries and TFP4 to the Golgi. TFP1 crucially contributes to parasite fitness and survival while the other members of this family are dispensable. Conditional depletion of TFP1 impairs microneme biogenesis and leads to a complete block in exocytosis, which hampers gliding motility, attachment, invasion and egress. Morphological investigations revealed that TFP1 participates in the condensation of the microneme content, suggesting the transport of a relevant molecule for maintaining the intraluminal microenvironment necessary for organelle maturation and exocytosis. In absence of TFP2, rhoptries adopt a considerable elongated shape, but the abundance, processing or secretion of the rhoptry content are not affected. These findings establish the relevance of TFPs in organelle maturation of T. gondii. This article is protected by copyright. All rights reserved.
PMID: 29738095
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Article (Published version) (19.5 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research group Biologie d'un parasite intracellulaire obligatoire (773)
Projects FNS: FN3100A0-116722, IZRJZ3_164183
ERC 695596
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HAMMOUDI, Pierre-Mehdi et al. Toxoplasma gondii TFP1 is an essential transporter family protein critical for microneme maturation and exocytosis. In: Molecular Microbiology, 2018. doi: 10.1111/mmi.13981 https://archive-ouverte.unige.ch/unige:106552

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Deposited on : 2018-07-20

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