UNIGE document Doctoral Thesis
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Therapeutic protein aggregates: biophysical characterization and in vitro modelisation of subcutaneous tissue

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Defense Thèse de doctorat : Univ. Genève, 2018 - Sc. 5172 - 2018/01/17
Abstract The aim of this thesis was to explore the link between stability and immunogenicity of therapeutic proteins. Using various environmental stress conditions, protein aggregates were formed, biophysically characterized, and the relationship between the degree of aggregation and the impact on the protein bioactivity highlighted. Then, in view of simulating in vitro the phenomena of in situ protein aggregation once injected in the subcutaneous tissue, we reviewed the literature on 2D and 3D assays predicting the potential immunogenicity of (new candidate) therapeutic proteins. Based on that, an immunocompetent model of the human subcutaneous tissue was developed, combining hydrogels scaffolds into which antigen-presenting cells (MUTZ-3 dendritic cells) were embedded.
Keywords Therapeutic proteinAggregatesImmunogenicitySubcutaneous tissue
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URN: urn:nbn:ch:unige-1032135
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Thesis (7.2 MB) - private document Private access (until 2018-09-23)
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Research group Biopharmacie
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GROELL, Floriane. Therapeutic protein aggregates: biophysical characterization and in vitro modelisation of subcutaneous tissue. Université de Genève. Thèse, 2018. https://archive-ouverte.unige.ch/unige:103213

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Deposited on : 2018-03-26

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