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Scientific article
English

A simple method for organotypic cultures of nervous tissue

Contributors

Stoppini, Luc; Buchs, Pierre-Alain; Muller, Dominique

Published in

Journal of neuroscience methods, vol. 37, no. 2, p. 173-182

Publication date

1991

Abstract

Hippocampal slices prepared from 2-23-day-old neonates were maintained in culture at the interface between air and a culture medium. They were placed on a sterile, transparent and porous membrane and kept in petri dishes in an incubator. No plasma clot or roller drum were used. This method yields thin slices which remain 1-4 cell layers thick and are characterized by a well preserved organotypic organization. Pyramidal neurons labelled by extra- and intracellular application of horse radish peroxidase resemble by the organization and complexity of their dendritic processes those observed in situ at a comparable developmental stage. Excitatory and inhibitory synaptic potentials can easily be analysed using extra- or intracellular recording techniques. After a few days in culture, long-term potentiation of synaptic responses can reproducibly be induced. Evidence for a sprouting response during the first days in culture or following sections is illustrated. This technique may represent an interesting alternative to roller tube cultures for studies of the developmental changes occurring during the first days or weeks in culture.

Keywords

  • Animals
  • Animals, Newborn/physiology
  • Culture Techniques
  • Electrophysiology
  • Fluorescent Antibody Technique
  • Glial Fibrillary Acidic Protein/analysis
  • Hippocampus/cytology
  • Horseradish Peroxidase/diagnostic use
  • Interneurons/cytology
  • Nervous System/ cytology
  • Pyramidal Tracts/cytology
  • Rats
  • Rats, Inbred Strains
  • Staining and Labeling
  • Synapses/drug effects

UNIGE affiliation entities

Citation (ISO format)

STOPPINI, Luc, BUCHS, Pierre-Alain, MULLER, Dominique. A simple method for organotypic cultures of nervous tissue. In: Journal of neuroscience methods, 1991, vol. 37, n° 2, p. 173–182. doi: 10.1016/0165-0270(91)90128-M

Identifiers

Additional URL for this publication

http://www.sciencedirect.com/science/article/pii/016502709190128M

Journal ISSN

0165-0270
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Technical informations

Creation

06/08/2010 10:47:25

First validation

06/08/2010 10:47:25

Update

14/03/2023 15:57:36

Status update

14/03/2023 15:57:36

Last indexation

29/10/2024 16:25:40
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