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Direct inhibition by opioid peptides of neurones located in the ventromedial nucleus of the guinea pig hypothalamus

Charpak, S.
Dreifuss, Jean-Jacques
Published in Brain Research. 1988, vol. 450, no. 1-2, p. 124-130
Abstract In slices of guinea pig brain, intracellular recordings were obtained from neurones of the ventromedial nucleus of the hypothalamus (VMH). [D-Ala2,NMePhe4,Gly-ol5]enkephalin (DAGO), an agonist selective for mu-opioid receptors, caused an inhibition of spontaneous firing activity and a membrane hyperpolarization. This effect was reversible, concentration-dependent and could be blocked by naloxone. DAGO directly inhibited VMH neurones since its effect persisted when the slice was perifused with a solution that blocks synaptic transmission. The hyperpolarization induced by DAGO was associated with a marked decrease in membrane input resistance and it was reversed in polarity at membrane potentials 30-40 mV more negative than the resting potential. A chloride current did not contribute to the hyperpolarization brought about by DAGO. We conclude that DAGO inhibits VMH neurones, probably by opening membrane potassium channels.
Keywords Action Potentials/drug effectsAnimalsEnkephalin, Ala(2)-MePhe(4)-Gly(5)-Enkephalin, D-Penicillamine (2,5)-Enkephalins/ pharmacologyGuinea PigsMaleMembrane Potentials/drug effectsNeural Inhibition/ drug effectsVentromedial Hypothalamic Nucleus/ drug effects/physiology
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PMID: 3401706

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Deposited on : 2010-08-06

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