Scientific article

Validation of an ultra-fast UPLC-UV method for the separation of antituberculosis tablets

Published inJournal of separation science, vol. 31, p. 1050-1056
Publication date2008

A simple method using ultra performance LC (UPLC) coupled with UV detection was developed and validated for the determination of antituberculosis drugs in combined dosage form, i. e. isoniazid (ISN), pyrazinamide (PYR) and rifampicin (RIF). Drugs were separated on a short column (2.1 mmx50 mm) packed with 1.7 mum particles, using an elution gradient procedure. At 30 degrees C, less than 2 min was necessary for the complete separation of the three antituberculosis drugs, while the original USP method was performed in 15 min. Further improvements were obtained with the combination of UPLC and high temperature (up to 90 degrees C), namely HT-UPLC, which allows the application of higher mobile phase flow rates. Therefore, the separation of ISN, PYR and RIF was performed in less than 1 min. After validation (selectivity, trueness, precision and accuracy), both methods (UPLC and HT-UPLC) have proven suitable for the routine quality control analysis of antituberculosis drugs in combined dosage form. Additionally, a large number of samples per day can be analysed due to the short analysis times.

  • Antituberculosis
  • Fast-LC
  • High temperature
  • Method validation
Citation (ISO format)
NGUYEN, Dao Thu-Trang et al. Validation of an ultra-fast UPLC-UV method for the separation of antituberculosis tablets. In: Journal of separation science, 2008, vol. 31, p. 1050–1056. doi: 10.1002/jssc.200700537
Main files (1)
Article (Published version)
ISSN of the journal1615-9306

Technical informations

Creation10/29/2008 11:37:29 AM
First validation10/29/2008 11:37:29 AM
Update time03/14/2023 2:56:26 PM
Status update03/14/2023 2:56:25 PM
Last indexation01/16/2024 10:24:55 AM
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