An amylase/Cre transgene marks the whole endoderm but the primordia of liver and ventral pancreas

Article Article scientifique Article An amylase/Cre transgene marks the whole endoderm but the primordia of liver and ventral pancreas eng 2006 author Kockel Liliana 507259 author Strom Alessandra 543744 author Delacour Alexandra 547047 author Nepote Virginie 547132 author Hagenbuchle Otto author Wellauer P. K. author Herrera Pedro Luis 0000-0003-0771-9504 23981 287-296 10.1002/dvg.20206 16786601 1526-954X Mice bearing a Cre-encoding transgene driven by a compound [SV40 small t antigen/mousealpha-amylase-2] promoter expressed the recombinase at early developmental stages broadly in the embryonic endoderm before the pancreas and lungs begin to outgrow, but not in other germ layers, as determined indirectly by beta-galactosidase and YFP reporter activity, indicating that the transgene is in fact an endodermic marker. Interestingly, the liver and ventral pancreas were excluded from this expression pattern, denoting that the chimerical alpha-amylase-2 promoter was not active in the anterior leading edge of the endoderm (the presumptive region from which liver and ventral pancreas form). These transgenics thus confirm, among other findings, that dorsal and ventral pancreatic primordia have different intrinsic transcriptional capabilities. In conclusion, we have generated a new transgenic mouse that should be useful to target endoderm at early stages, without affecting the liver or ventral pancreas before embryonic day E12.5. Animals Antigens, Polyomavirus Transforming/genetics Cell Lineage Endocrine System/embryology/metabolism Endoderm/ metabolism Gene Expression Homeodomain Proteins/genetics Humans Integrases/ genetics Liver/ embryology/metabolism Lymph Nodes/metabolism Mice Mice, Transgenic/ embryology Models, Genetic Pancreas/ embryology/metabolism Salivary Glands/metabolism Stem Cells/metabolism Transcription Factors/genetics Transgenes Alpha-Amylases/ genetics Dewey 616 56 Département de médecine 221 false 272 Types cellulaires pancréatiques pendant l'ontogénèse 522 Genesis 44 6 8f87fc92-6888-4728-a01f-1d12d5f19d2a Article Restricted file1.pdf 1010125 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine

Title	Published in	Access level	OA Policy	Year	Views	Downloads

An amylase/Cre transgene marks the whole endoderm but the primordia of liver and ventral pancreas	Genesis			2006	659	0

Archive ouverte UNIGE

Kockel, Liliana