A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology

Article Article scientifique Article A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology eng 1998-09 author Correges Philippe 27701 author Bugnard Elisabeth 503986 author Millerin C. author Masiero Agostino 25995 author Andrivet Jean-Pierre 25821 author Bloc Alain André Leon 99264 author Dunant Yves 25134 177-184 10.1016/S0165-0270(98)00079-X 0165-0270 https://linkinghub.elsevier.com/retrieve/pii/S016502709800079X Most of the parameters recorded in electrophysiology are strongly temperature dependent. In order to control temperature fluctuations we have built a system that ensures an accurate thermoregulation of the recording chamber. Temperature of physiological preparations can be changed relatively quickly (about 8°C/min) and with a good accuracy (±0.5°C) without inducing thermal oscillations. Contrary to other thermoregulating devices, the temperature regulation is not carried out through the perfused medium but directly at the bottom of the chamber where a 3-cm2 Peltier element has been placed. The element is driven by a dedicated electronic device which controls the amount and the direction of the current flowing across the Peltier thermocouple. All construction details and the appropriate electrical circuits are provided. Using this home-made device, the steady-state chamber temperature could be precisely monitored with a resolution of ±0.1°C in a range of 0–40°C. This set-up was tested in experiments designed to evaluate the temperature dependence of synaptic transmission in the Torpedo nerve–electroplate synapses and of calcium currents recorded from isolated nerve cells. This low-cost method is suitable for a wide range of applications. Electrophysiology Peltier effect Temperature control Synaptic transmission Calcium channel Dewey 615 Dewey 617 31 Section des sciences pharmaceutiques 17 63 Département d'anesthésiologie, pharmacologie, soins intensifs et urgences 229 false Journal of Neuroscience Methods 83 2 da2e96ec-b3fc-4aa7-8f7a-417e20549f89 Article (Published version) Restricted 1-s2.0-S016502709800079X-main.pdf 184455 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences

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A simple, low-cost and fast Peltier thermoregulation set-up for electrophysiology	Journal of Neuroscience Methods			1998	66	0

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Bugnard, Elisabeth