Guillarme, Davy

 
 
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2021

Scientific Articles

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Algorithms to optimize multi-column chromatographic separations of proteinsCodesido Sanchez, Santiago; Guillarme, Davy; Fekete, Szabolcs;
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Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometryLardeux, Honorine; Duivelshof, Bastiaan L.; Colas, Olivier; Beck, Alain; ... D'Atri, Valentina
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Analytical challenges encountered and the potential of supercritical fluid chromatography: A perspective of five expertsOlesik, Susan; West, Caroline; Guillarme, Davy; Mangelings, Debby; Novakova, Lucie
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Aptamer-based immunoaffinity LC-MS using an ultra-short column for rapid attomole level quantitation of intact mAbsNguyen, Jennifer M; Liu, Xiaoxiao; DeLoffi, Maureen; Murisier, Amarande; ... Lauber, Matthew A
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Bispecific antibody characterization by a combination of intact and site-specific/chain-specific LC/MS techniquesDuivelshof, Bastiaan; Debecker, Alain; Guillarme, Davy; D'Atri, Valentina;
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Empirical correction of non-linear pH gradients and a tool for application to protein ion exchange chromatographyFekete, Szabolcs; Murisier, Amarande; Lauber, Matthew; Guillarme, Davy;
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Enantiomeric methadone quantitation on real post-mortem dried matrix spots samples: Comparison of liquid chromatography and supercritical fluid chromatography coupled to mass spectrometryMueller, Françoise; Losacco, Gioacchino Luca; Nicoli, Raul; Guillarme, Davy; ... Grata, E
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Expanding the range of sub/supercritical fluid chromatography: Advantageous use of methanesulfonic acid in water-rich modifiers for peptide analysisLosacco, Gioacchino Luca; Da Silva, Jimmy Oliviera; Liu, Jinchu; Regalado, Erik L; ... Guillarme, Davy
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Free Excel Software for Performing Virtual Liquid ChromatographyGuillarme, Davy; Balazs, Bobably; Veuthey, Jean-Luc;
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Inter-laboratory study to evaluate the performance of automated online characterization of antibody charge variants by multi-dimensional LC-MS/MSCamperi, Julien; Grunert, Ingrid; Heinrich, Katrin; Winter, Martin; ... Stella, Cinzia
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Interlaboratory study of a supercritical fluid chromatography method for the determination of pharmaceutical impurities: Evaluation of multi-systems reproducibilityDispas, Amandine; Clarke, Adrian; Grand-Guillaume Perrenoud, Alexandre; Losacco, Gioacchino Luca; ... Hubert, Philippe
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Ion mobility-high resolution mass spectrometry in anti-doping analysis. Part I: Implementation of a screening method with the assessment of a library of substances prohibited in sportsPlachká, Kateřina; Pezzatti, Julian; Musenga, Alessandro; Nicoli, Raul; ... Guillarme, Davy
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Ion mobility-high resolution mass spectrometry in doping control analysis. Part II: Comparison of acquisition modes with and without ion mobilityPlachká, Kateřina; Pezzatti, Julian; Musenga, Alessandro; Nicoli, Raul; ... Guillarme, Davy
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Isolation and identification of isocoumarin derivatives with specific inhibitory activity against wnt pathway and metabolome characterization of lasiodiplodia venezuelensisPellissier, Léonie; Koval, Alexey; Marcourt, Laurence; Ferreira Queiroz, Emerson; ... Wolfender, Jean-Luc
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Metamorphosis of supercritical fluid chromatography: A viable tool for the analysis of polar compounds?Losacco, Gioacchino Luca; Veuthey, Jean-Luc; Guillarme, Davy;
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Multi-dimensional LC-MS: the next generation characterization of antibody-based therapeutics by unified online bottom-up, middle-up and intact approachesCamperi, Julien; Goyon, Alexandre; Guillarme, Davy; Zhang, Kelly; Stella, Cinzia
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Negative gradient slope methods to improve the separation of closely eluting proteinsFekete, Szabolcs; Murisier, Amarande; Nguyen, Jennifer M.; Lauber, Matthew A.; Guillarme, Davy
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New wide-pore superficially porous stationary phases with low hydrophobicity applied for the analysis of monoclonal antibodiesFekete, Szabolcs; Murisier, Amarande; Beck, Alain; Lawhorn, Jason; ... Guillarme, Davy
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Quantitative N-Glycan Profiling of Therapeutic Monoclonal Antibodies Performed by Middle-Up Level HILIC-HRMS AnalysisDuivelshof, Bastiaan; Denorme, Steffy; Sandra, Koen; Liu, Xiaoxiao; ... D'Atri, Valentina
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State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates SynthesisDeslignière, Evolène; Ehkirch, Anthony; Duivelshof, Bastiaan; Toftevall, Hanna; ... Cianférani, Sarah
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The importance of being metal-free: The critical choice of column hardware for size exclusion chromatography coupled to high resolution mass spectrometryMurisier, Amarande; Fekete, Szabolcs; Guillarme, Davy; D'Atri, Valentina;
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Therapeutic Fc‐fusion proteins: Current analytical strategiesDuivelshof, Bastiaan; Murisier, Amarande; Camperi, Julien; Fekete, Szabolcs; ... D'Atri, Valentina
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Towards a simple on-line coupling of ion exchange chromatography and native mass spectrometry for the detailed characterization of monoclonal antibodiesMurisier, Amarande; Duivelshof, Bastiaan; Fekete, Szabolcs; Bourquin, Julien; ... D'Atri, Valentina
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Ultra-short ion-exchange columns for fast charge variants analysis of therapeutic proteinsNavarro-Huerta, Jose Antonio; Murisier, Amarande; Nguyen, Jennifer M.; Lauber, Matthew A.; ... Fekete, Szabolcs
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Use of Ultra-short Columns for Therapeutic Protein Separations, Part 2: Designing the Optimal Column Dimension for Reversed-Phase Liquid ChromatographyFekete, Szabolcs; Murisier, Amarande; Nguyen, Jennifer M; Bolton, Michael J.; ... Guillarme, Davy
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Use of Ultrashort Columns for Therapeutic Protein Separations. Part 1: Theoretical Considerations and Proof of ConceptFekete, Szabolcs; Bobaly, Balazs; Nguyen, Jennifer M; Beck, Alain; ... Guillarme, Davy
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Using 1.5 mm internal diameter columns for optimal compatibility with current liquid chromatographic systemsFekete, Szabolcs; Murisier, Amarande; Losacco, Gioacchino Luca; Lawhorn, Jason; ... Guillarme, Davy

Professional Articles

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Biologics Explorer - Sitting Down With… Davy Guillarme, Senior Lecturer and Research Associate, School of Pharmaceutical Sciences, University of Geneva, SwitzerlandGuillarme, Davy;

Book Chapters

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Characterization of Glycosylated Proteins at Subunit Level by HILIC/MSD'Atri, Valentina; Guillarme, Davy;
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Fast Afucosylation Profiling of Glycoengineered Antibody Subunits by Middle-Up Mass SpectrometryWagner-Rousset, Elsa; Colas, Olivier; Chenu, Stéphane; François, Yannis-Nicolas; ... Beck, Alain

Thesis supervisions

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Evolution of sub/supercritical fluid chromatography – mass spectrometry for the analysis of highly polar compounds and biological matricesLosacco, Gioacchino Luca;

2020

Scientific Articles

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Applicability of Supercritical fluid chromatography–Mass spectrometry to metabolomics. II–Assessment of a comprehensive library of metabolites and evaluation of biological matricesLosacco, Gioacchino Luca; Ismail, Omar; Pezzatti, Julian; Gonzalez Ruiz, Victor; ... Guillarme, Davy
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Automated middle-up approach for the characterization of biotherapeutic products by combining on-line hinge-specific digestion with RPLC-HRMS analysisCamperi, Julien; Guillarme, Davy; Lei, Ming; Stella, Cinzia;
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Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and related productsFarsang, Evelin; Guillarme, Davy; Veuthey, Jean-Luc; Beck, Alain; ... Fekete, Szabolcs
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Current and future trends in reversed-phase liquid chromatography-mass spectrometry of therapeutic proteinsD'Atri, Valentina; Murisier, Amarande; Fekete, Szabolcs; Veuthey, Jean-Luc; Guillarme, Davy
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Determination of size variants by CE-SDS for approved therapeutic antibodies: Key implications of subclasses and light chain specificitiesWagner, Elsa; Colas, Olivier; Chenu, Stéphane; Goyon, Alexandre; ... Beck, Alain
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Development of a 3D-LC/MS Workflow for Fast, Automated, and Effective Characterization of Glycosylation Patterns of Biotherapeutic ProductsCamperi, Julien; Dai, Lu; Guillarme, Davy; Stella, Cinzia;
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Development of an innovative salt-mediated pH gradient cation exchange chromatography method for the characterization of therapeutic antibodiesGoyon, Alexandre; McDonald, Daniel; Fekete, Szabolcs; Guillarme, Davy; Stella, Cinzia
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Editorial for the special issue titled "Biopharmaceuticals 2020"Fekete, Szabolcs; Guillarme, Davy;
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Editorial for the virtual special issue SEP 2019Guillarme, Davy; West, Caroline;
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Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phaseMcCalley, David V.; Guillarme, Davy;
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Evaluation of Different Tandem MS Acquisition Modes to Support Metabolite Annotation in Human Plasma Using Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometry for Untargeted MetabolomicsPezzatti, Julian; Gonzalez Ruiz, Victor; Boccard, Julien; Guillarme, Davy; Rudaz, Serge
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Fast and Automated Characterization of Monoclonal Antibody Minor Variants from Cell Cultures by Combined Protein-A and Multidimensional LC/MS MethodologiesCamperi, Julien; Dai, Lu; Guillarme, Davy; Stella, Cinzia;
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From proof of concept to the routine use of an automated and robust multi-dimensional liquid chromatography mass spectrometry workflow applied for the charge variant characterization of therapeutic antibodiesGoyon, Alexandre; Dai, Lu; Chen, Tao; Wei, Bingchuan; ... Stella, Cinzia
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Glycan-Mediated Technology for Obtaining Homogeneous Site-Specific Conjugated Antibody-Drug Conjugates: Synthesis and Analytical Characterization by Using Complementary Middle-up LC/HRMS AnalysisDuivelshof, Bastiaan; Deslignière, Evolène; Hernandez-Alba, Oscar; Ehkirch, Anthony; ... D'Atri, Valentina
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Impact of the column on effluent pH in cation exchange pH gradient chromatography, a practical studyFarsang, Evelin; Horváth, Krisztián; Beck, Alain; Wang, Qi; ... Fekete, Szabolcs
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