OBJECTIVE: The aim of this in vitro pilot study was to evaluate the influence of an artificial saliva (AS) lubricant on the retentive force of a stud-type attachment (LOCATOR(R) ) for implant overdentures (IODs). METHODS: Twenty custom-made models simulating a two-IOD with parallel implant situation were fabricated using LOCATOR(R) attachments. The in vitro testing was carried out with an Instron(R) universal testing machine for a total of 10,000 insertion-removal cycles, for each model, in two different aqueous test mediums (Group 1: 0.9% sodium chloride solution (NaCl), n = 10; Group 2: AS, n = 10). Changes in the mean retentive force (F) were plotted against the cycle numbers #10, #100, #1000, #5000, and #10,000. Mixed regression models were applied for statistical analyses. RESULTS: A mixed regression (not considering interactions) predicted, compared to cycle #10, higher retentive forces at cycles #100 (P < 0.0001), #1000 (P = 0.017), similar forces at #5000 (P = 0.277), and lower forces at #10,000 (P = 0.012); there was no overall effect of the medium (P = 0.159). A second statistical model, employing the interaction term "cycle##medium", confirmed similarly the effect. Although the interaction term was significant at cycle #100 (p = 0.045), there was no significant difference between the two groups (P = 0.140). CONCLUSION: In this in vitro pilot experiment, there was no difference in mean retentive forces of the LOCATOR(R) attachments when tested with either 0.9% NaCl or a Glandosane(R) -like artificial saliva lubricant. A larger scale study may still confirm the superiority of either lubricant for quasiclinical bench experiments.