From the leaves of Withania adpressa, a plant endemic to Sahara of Morocco and Algeria, the novel steroidal lactone (22R)-14 alpha;,15 alpha;,17ß,20ß-tetrahydroxy-1-oxowitha-2,5,24-trien-26,22-olide (= (15S,17S)-14,15,17,20-tetrahydroxy-22,26-epoxyergosta-2,5,24-triene-1,26-dione; 1), was isolated, along with three known compounds, withanolides F (2), J (3), and oleanolic acid. Their structures were mainly solved by in-depth 1D- and 2D-NMR (including ADEQUATE) experiments, as well as by HR-MS analyses and chemical evidence.