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Scientific article
English

Concentration of GPI-anchored proteins upon ER exit in yeast

Published inTraffic, vol. 10, no. 2, p. 186-200
Publication date2009
Abstract

Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p.

Keywords
  • Amino Acid Transport Systems/genetics/metabolism
  • Cell Wall/genetics/*metabolism
  • Cytoplasmic Vesicles/metabolism
  • Endoplasmic Reticulum/*metabolism
  • Glucose Transport Proteins, Facilitative
  • Glycosylphosphatidylinositols/*metabolism
  • Membrane Glycoproteins/genetics/*metabolism
  • Membrane Proteins/genetics/metabolism
  • Monosaccharide Transport Proteins/genetics/metabolism
  • Saccharomyces cerevisiae/genetics/*metabolism
  • Saccharomyces cerevisiae Proteins/genetics/*metabolism
  • Temperature
  • Vesicular Transport Proteins/genetics/metabolism
Citation (ISO format)
CASTILLON, Guillaume Alain et al. Concentration of GPI-anchored proteins upon ER exit in yeast. In: Traffic, 2009, vol. 10, n° 2, p. 186–200. doi: 10.1111/j.1600-0854.2008.00857.x
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ISSN of the journal1398-9219
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