The lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) is known to self-assemble into single-wall synthetic lipid nanotubes (LNTs) on polyelectrolyte-functionalized surfaces. We have demonstrated a high-throughput approach to fabricate gold nanowires on surfaces with a LNT template. First, biotin-tagged DOPE LNTs are formed from lipid blocks in inverted hexagonal phase adsorbed on polymer-coated surfaces upon application of shear force. Streptavidin-coated gold nanoparticles (AuNPs) were then attached to the biotin-tagged LNTs and AuNP-encapsulated LNTs were cross-linked by chemical fixation. Samples were dried and AuNPs were connected through electroless gold metal plating to form gold nanowires. Similarly, the created LNTs without the attachment of AuNPs were adsorbed on polymer-coated surfaces and cross-linked by chemical fixation. These were dried and treated with high temperature in a process of pyrolysis under inert atmosphere to form connected carbon nanostructures. The created nanowires and carbon nanostructures were characterized by transmission electron microscopy, atomic force microscopy and electrical measurements.