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Title

RIG-I and dsRNA-induced IFNbeta activation

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Published in PLOS ONE. 2008, vol. 3, no. 12, p. e3965
Abstract Except for viruses that initiate RNA synthesis with a protein primer (e.g., picornaviruses), most RNA viruses initiate RNA synthesis with an NTP, and at least some of their viral (ppp)RNAs remain unblocked during the infection. Consistent with this, most viruses require RIG-I to mount an innate immune response, whereas picornaviruses require mda-5. We have examined a SeV infection whose ability to induce interferon depends on the generation of capped dsRNA (without free 5' tri-phosphate ends), and found that this infection as well requires RIG-I and not mda-5. We also provide evidence that RIG-I interacts with poly-I/C in vivo, and that heteropolymeric dsRNA and poly-I/C interact directly with RIG-I in vitro, but in different ways; i.e., poly-I/C has the unique ability to stimulate the helicase ATPase of RIG-I variants which lack the C-terminal regulatory domain.
Keywords AnimalsBinding SitesCells, CulturedDEAD-box RNA Helicases/genetics/metabolismGreen Fluorescent Proteins/genetics/metabolismInterferon-beta/immunology/metabolismMicePoly I-C/geneticsProtein Structure, TertiaryRNA, Double-Stranded/metabolismSendai virus/genetics/metabolismTransfectionViral Proteins/genetics/metabolism
Stable URL http://archive-ouverte.unige.ch/unige:1290
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Identifiers
PMID: 19115016
Structures
Research group Virologie moléculaire (275)

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Deposited on : 2009-04-01

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