en
Scientific article
English

Multiple injection technique for the determination and quantitation of insulin formulations by capillary electrophoresis and time-of-flight mass spectrometry

Published inJournal of chromatography, vol. 1217, no. 51, p. 8041-8047
Publication date2010
Abstract

This paper describes an efficient CE-UV-ESI-TOF/MS method for the determination and quantitation of intact insulin (INS) in a pharmaceutical formulation. The CE conditions were optimized to avoid the adsorption of proteins onto the capillary wall. Particular attention was paid regarding the choice of the internal standard (IS). A strategy based on multiple injections was selected and the methodology was validated according to international guidelines. The optimized method was applied with success to the analysis of INS formulations obtained from regular and parallel markets.

Keywords
  • Capillary electrophoresis
  • Intact protein
  • Multiple injection
  • Quantitation
  • Time-of-flight mass spectrometry
Citation (ISO format)
STAUB SPORRI, Aline et al. Multiple injection technique for the determination and quantitation of insulin formulations by capillary electrophoresis and time-of-flight mass spectrometry. In: Journal of chromatography, 2010, vol. 1217, n° 51, p. 8041–8047. doi: 10.1016/j.chroma.2010.08.076
Main files (1)
Article (Accepted version)
accessLevelRestricted
Identifiers
ISSN of the journal0021-9673
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Technical informations

Creation11/25/2010 10:05:00 AM
First validation11/25/2010 10:05:00 AM
Update time03/14/2023 4:09:34 PM
Status update03/14/2023 4:09:34 PM
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