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Scientific article
English

Quantification of cyclosporine and tacrolimus in whole blood. Comparison of liquid chromatography-electrospray mass spectrometry with the enzyme multiplied immunoassay technique

Published inClinical biochemistry, vol. 41, no. 10-11, p. 910-913
Publication date2008
Abstract

OBJECTIVES: The aim of this work was to compare a validated liquid chromatography-mass spectrometry (LC-MS) method with the commercial enzyme multiplied immunoassay technique (EMIT) for cyclosporine and tacrolimus whole blood quantification. DESIGN AND METHODS: Samples of transplant patients receiving cyclosporine (n=38) or tacrolimus (n=41) were analyzed successively by LC-MS and EMIT. Several statistical approaches for method comparison were evaluated and Passing-Bablok and Bland-Altman analyses chosen. RESULTS: Overestimations of the concentrations measured with EMIT compared to LC-MS were observed with means of 23% (range: 6% to 46%) for cyclosporine and 30% (range: -3% to 73%) for tacrolimus. CONCLUSION: The EMIT demonstrated significant positive biases due to cross-reactions with metabolites. This indicates that, in some clinical situations, a selective method such as LC-MS is preferable for therapeutic drug monitoring in transplant patients.

Keywords
  • Cross Reactions
  • Cyclosporine/blood
  • Enzyme Multiplied Immunoassay Technique
  • Humans
  • Immunoassay
  • Spectrometry, Mass, Electrospray Ionization/methods
  • Tacrolimus/blood
  • Transplantation Immunology
Citation (ISO format)
ANSERMOT, Nicolas et al. Quantification of cyclosporine and tacrolimus in whole blood. Comparison of liquid chromatography-electrospray mass spectrometry with the enzyme multiplied immunoassay technique. In: Clinical biochemistry, 2008, vol. 41, n° 10-11, p. 910–913. doi: 10.1016/j.clinbiochem.2008.02.015
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ISSN of the journal1873-2933
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