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Control of ribosome biogenesis by the TORC1-Sch9 pathway in Saccharomyces cerevisiae

Defense Thèse de doctorat : Univ. Genève, 2010 - Sc. 4243 - 2010/08/20
Abstract Yeast TORC1 is a evolutionarily conserved multiprotein complex whose kinase activity is ensured by the TOR catalytic subunit. In response to environmental cues, TORC1 directly phosphorylates and activates the AGC kinase Sch9 to regulate anabolic processes such as translation. We show here that Sch9 mediates TORC1 signals to all three nuclear RNA polymerases (RNAP I-III) via transcriptional repressors. Maf1 is directly phosphorylated an inhibited by Sch9 to prevent it from repressing RNAP III. Stb3, Dot6 and Tod6 are also directly phosphorylated by Sch9 to keep them from recruiting RPD3L upstream of ribosome biogenesis and ribosomal protein genes and thereby from repressing their transcription by RNAP II. In turn, expression of those genes serves to promote RNAP I activity.
Keywords BuddingYeastTORRapamycinSch9KinaseTranscription factorRNAPolymeraseRNAPIRNAPIIIRNAPIIRNA Pol IRNA Pol IIRNA Pol IIIMaf1Stb3Dot6Tod6Rpd3RPD3LHistoneDeacetylasePhosphorylationCell sizeRibosomeRibosome biogenesisTranslationTranscriptionRibiRPSaccharpomycesCerevisiaeSignalingSignallingTORC1AGC kinaseGrowth
Stable URL http://archive-ouverte.unige.ch/unige:11871
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URN: urn:nbn:ch:unige-118715
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