UNIGE document Scientific Article
previous document  unige:1186  next document
add to browser collection

Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis

Yoshinobu, Kumiko
Fossati-Jimack, Liliane
Morley, Bernard J.
Vyse, Timothy J.
Hirose, Sachiko
show hidden authors show all authors [1 - 14]
Published in Journal of Immunology. 2008, vol. 181, no. 4, p. 2846-54
Abstract The endogenous retroviral envelope glycoprotein, gp70, implicated in murine lupus nephritis is secreted by hepatocytes as an acute phase protein, and it has been thought to be a product of an endogenous xenotropic virus, NZB-X1. However, since endogenous polytropic (PT) and modified polytropic (mPT) viruses encode gp70s that are closely related to xenotropic gp70, these viruses can be additional sources of serum gp70. To better understand the genetic basis of the expression of serum gp70, we analyzed the abundance of xenotropic, PT, or mPT gp70 RNAs in livers and the genomic composition of corresponding proviruses in various strains of mice, including two different Sgp (serum gp70 production) congenic mice. Our results demonstrated that the expression of different viral gp70 RNAs was remarkably heterogeneous among various mouse strains and that the level of serum gp70 production was regulated by multiple structural and regulatory genes. Additionally, a significant contribution of PT and mPT gp70s to serum gp70 was revealed by the detection of PT and mPT, but not xenotropic transcripts in 129 mice, and by a closer correlation of serum levels of gp70 with the abundance of PT and mPT gp70 RNAs than with that of xenotropic gp70 RNA in Sgp3 congenic mice. Furthermore, the injection of lipopolysaccharides selectively up-regulated the expression of xenotropic and mPT gp70 RNAs, but not PT gp70 RNA. Our data indicate that the genetic origin of serum gp70 is more heterogeneous than previously thought, and that distinct retroviral gp70s are differentially regulated in physiological vs inflammatory conditions.
Keywords AnimalsAutoantigens/biosynthesis/genetics/immunologyEndogenous Retroviruses/genetics/immunologyGlycoproteins/biosynthesis/blood/geneticsInflammation Mediators/blood/physiologyLupus Nephritis/blood/genetics/pathologyMiceMice, CongenicMice, Inbred C57BLMice, Inbred MRL lprMice, Inbred NZBMolecular Chaperones/biosynthesis/genetics
Stable URL http://archive-ouverte.unige.ch/unige:1186
Full text
Article (Author postprint) (863 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jimmunol.org/cgi/content/full/181/4/2846
PMID: 18684976
Research group Lupus érythémateux systémique, de l'anémie hémolytique et de la glomérulonéphrite (168)
138 hits and 3 downloads since 2009-03-19
Export document
Format :
Citation style :