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Imaging mass spectrometry using peptide isoelectric focusing

Chauvet, Alexis
Published in Rapid Communications in Mass Spectrometry. 2008, vol. 22, no. 17, p. 2667-76
Abstract Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The use of Immobilized pH Gradient-IsoElectric Focusing (IPG-IEF) is also a new trend, as the first dimension of separation, in shotgun proteomics. We report a combination of these two outstanding technologies. This approach is based on the separation of shotgun-produced peptides by IPG-IEF. The peptides are then transferred by capillarity to a capture membrane, which is then scanned by the mass spectrometer to generate MS images. This high-throughput methodology allows a preview of the sample to be obtained in a single day. We report the application of this new pipeline for differential comparison of the membrane proteome of two different strains of Staphylococcus aureus bacteria in a proof-of-principle experiment.
Keywords Isoelectric FocusingMembrane Proteins/chemistryPeptide MappingProteomics/methodsSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation/methodsStaphylococcus aureus/chemistry/classificationTandem Mass Spectrometry/instrumentation/methods
Stable URL http://archive-ouverte.unige.ch/unige:1181
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PMID: 18677718
Research group La protéomique au service de la clinique (270)
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