UNIGE document Scientific Article
previous document  unige:1173  next document
add to browser collection

Mesenchymal stem cells derived from human exocrine pancreas express transcription factors implicated in beta-cell development

Serre-Beinier, Veronique
Published in Pancreas. 2008, vol. 37, no. 1, p. 75-84
Abstract OBJECTIVES: Transplantation of in vitro generated islets or insulin-producing cells represents an attractive option to overcome organ shortage. The aim of this study was to isolate, expand, and characterize cells from human exocrine pancreas and analyze their potential to differentiate into beta cells. METHODS: Fibroblast-like cells growing out of human exocrine tissue were characterized by flow cytometry and by their capacity to differentiate into mesenchymal cell lineages. During cell expansion and after differentiation toward beta cells, expression of transcription factors of endocrine pancreatic progenitors was analyzed by reverse transcription polymerase chain reaction. RESULTS: Cells emerged from 14/18 human pancreatic exocrine fractions and were expanded up to 40 population doublings. These cells displayed surface antigens similar to mesenchymal stem cells from bone marrow. A culture of these cells in adipogenic and chondrogenic differentiation media allowed differentiation into adipocyte- and chondrocyte-like cells. During expansion, cells expressed transcription factors implicated in islet development such as Isl1, Nkx2.2, Nkx6.1, nestin, Ngn3, Pdx1, and NeuroD. Activin A and hepatocyte growth factor induced an expression of insulin, glucagon, and glucokinase. CONCLUSIONS: Proliferating cells with characteristics of mesenchymal stem cells and endocrine progenitors were isolated from exocrine tissue. Under specific conditions, these cells expressed little insulin. Human pancreatic exocrine tissue might thus be a source of endocrine cell progenitors.
Keywords Adipocytes/metabolismBone Marrow Cells/metabolismCell Differentiation/geneticsCell LineageCell ProliferationCells, CulturedChondrocytes/metabolismFibroblasts/metabolismFlow CytometryFluorescent Antibody TechniqueHumansInsulin/metabolismInsulin-Secreting Cells/metabolismIntermediate Filament Proteins/metabolismMesenchymal Stem Cells/metabolismNerve Tissue Proteins/metabolismPancreas, Exocrine/growth & development/metabolismPhenotypeReverse Transcriptase Polymerase Chain ReactionTime FactorsTranscription Factors/genetics/metabolismVimentin/metabolism
Stable URL http://archive-ouverte.unige.ch/unige:1173
Full text
PMID: 18580448
Research groups Chirurgie viscérale (104)
Cultures cellulaires et transplantations (519)
La transplantation d'îlots de Langerhans (623)
149 hits and 1 download since 2009-03-19
Export document
Format :
Citation style :