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On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography–UV detection using post-column derivatization and liquid chromatography–mass spectrometry

Yariwake, Janete H.
Queiroz, Emerson Ferreira
Published in Journal of Chromatography. A. 2005, vol. 1082, no. 1, p. 51 - 59
Abstract Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization-MS/MS and collision-induced dissociation-MS). On-line UV and MS data demonstrated the presence of methoxyflavone glycosides and aglycones in a total of seven compounds. Three naturally occurring flavones glycosides and two unusual erythro- and threo-diastereoisomeric flavolignan 7-O-glucosides were identified together with their aglycones.
Keywords Saccharum officinarumLc/uvLc/msFlavonoidsTransgenic plants
Stable URL http://archive-ouverte.unige.ch/unige:11655
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