UNIGE document Scientific Article
previous document  unige:11645  next document
add to browser collection

Igh-C allotype-linked control of anti-DNA production and clonotype expression in mice infected with Plasmodium yoelii

Yoshida, Haruyoshi
Fossati-Jimack, Liliane
Yoshida, M.
Abdelmoula, M.
Herrera, S.
Merino, Jesus
Published in Journal of Immunology. 1988, vol. 141, no. 6, p. 2125-2131
Abstract The infection of nonlethal strain of Plasmodium yoelii induces the formation of IgG anti-DNA antibodies as a result of polyclonal B cell activation. By using various nonautoimmune strains of mice including H-2 or Igh congenic or recombinant mice, the levels and clonotypes of anti-DNA antibodies elicited by the malaria infection were analyzed in relation to the expression of the MHC or Igh gene. Our results showed there were little, if any, differences in serum anti-DNA levels and their clonotypes among B10 and B6 H-2 congenic mice. In contrast, malaria-induced IgG anti-DNA responses markedly differed quantitatively and clonotypically between murine strains bearing the Ighb allotype and those bearing the Igha, Ighj, Ighd, or Ighn allotype. The latter group of mice produced approximately 5 to 10 times more IgG anti-DNA antibodies than the former group of mice. Clonotypically, mice bearing the Ighb allotype developed high alkaline anti-DNA antibodies of pH 8.0 to 8.5, whereas non-Ighb mice failed to express such alkaline anti-DNA spectrotypes, and exhibited more neutral spectrotypes (pH 7.0 to 8.0). Studies on the Igh recombinant mice indicated that the observed quantitative and clonotypical differences in IgG anti-DNA production was not associated with the variable region, but with the constant region of the Igh gene complex. Our results have suggested that IgG anti-DNA responses occurring as a result of polyclonal B cell activation during the course of malaria infection markedly differs quantitatively and clonotypically among murine strains and appear to be controlled at least in part by the Igh-C gene or gene(s) closely linked to it.
Keywords AnimalsAntibodies, Antinuclear/ biosynthesis/isolation & purificationAntibodies, Protozoan/biosynthesisAntibody DiversityFemaleGenes, ImmunoglobulinH-2 Antigens/geneticsImmunoglobulin Allotypes/ geneticsImmunoglobulin Constant Regions/geneticsImmunoglobulin Heavy Chains/ geneticsIsoelectric FocusingLinkage (Genetics)Malaria/genetics/ immunologyMiceMice, Inbred AKRMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLMice, Inbred NZBPlasmodium yoelii/immunology
Stable URL http://archive-ouverte.unige.ch/unige:11645
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jimmunol.org/cgi/reprint/141/6/2125.pdf
PMID: 3139752
144 hits and 0 download since 2010-08-27
Export document
Format :
Citation style :